J Korean Med Sci.  2004 Aug;19(4):586-590. 10.3346/jkms.2004.19.4.586.

Effect of DA-8159, a Selective Phosphodiesterase Type 5 Inhibitor, on Electroretinogram and Retinal Histology in Rabbits

• Cho HK
• Kang KK
• Ahn GJ
• Shim HJ
• Kim WB

• KMID: 2157690
• DOI: http://doi.org/10.3346/jkms.2004.19.4.586

Abstract

DA-8159, a selective inhibitor of phosphodiesterase type 5, was developed as a new drug for erectile dysfunction. The effect of DA-8159 on the electroretinogram (ERG) and the retinal histopathology were evaluated in rabbits. The ERG was performed prior to, and 1 and 5 hr after DA-8159 (5 to 30 mg/kg) administration. The plasma concentration of DA-8159 was determined at each time point, and retinal microscopic examination was also performed. There was no statistically significant ERG change at any dose or at any time. Though the 30 Hz flicker showed a prolongation of the implicit time at 5 hr after the administration of either DA-8159 15 mg or 30 mg/kg (p<0.05), but concurrent amplitude decreases were not statistically significant. At a dose of 5 mg/kg, no test drug was detected in the blood after either 1 or 5 hr. At either 15 mg/kg or 30 mg/kg, there was a dose-dependent increase in the blood concentration after 1 hr of drug administration, which decreased with time. In light and electron microscopic examinations of the retina, there was no remarkable change at any dose. These results suggest DA-8159 has a low risk potential to the retina, but further evaluation on the visual functions in human is needed.

Keyword

Electroretinography; Phosphodiesterase inhibitors; Rabbits; Retina

MeSH Terms

3',5'-Cyclic-GMP Phosphodiesterase/*antagonists & inhibitors
Animals
Dose-Response Relationship, Drug
Electroretinography/*drug effects
Humans
Male
Phosphodiesterase Inhibitors/blood/*pharmacology
Pyrimidines/blood/*pharmacology
Rabbits
Retina/*cytology/*drug effects

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