J Korean Med Sci.  2004 Aug;19(4):523-528. 10.3346/jkms.2004.19.4.523.

Homing-Associated Cell Adhesion Molecules and Cell Cycle Status on the Nucleated Cells in the Bone Marrow, Mobilized Peripheral Blood and Cord Blood

• Lee YH
• Lee YA
• Noh KT
• Kim KH
• Han JY
• Seo SY
• Kwon HC
• Kim JS
• Kim HJ

• KMID: 2157680
• DOI: http://doi.org/10.3346/jkms.2004.19.4.523

Abstract

Homing-associated cell adhesion molecules (H-CAM) on the CD34+ cells play an important role for the engraftment process following hematopoietic stem cell transplantation (HSCT). However, it seems that not only CD34+ cells but also other nucleated cells (NCs) with H-CAM could be implicated in the engraftment process and the proliferation of hematopoietic stem cells. We investigated the differences of HCAM and cell cycle status on the NCs in cord blood (CB), bone marrow (BM), and mobilized peripheral blood (PB). The proportions of CXCR4+ cells within the NC populations were greater in CB than in PB or BM (p=0.0493), although the proportions of CXCR4+, CD44+, and CD49d+ cells within the CB CD34+ cell populations were same within BM or PB. A lower proportion of CD34+CD49d+ cells within the CD34+ cell populations was more noted in CB than in PB or BM (p=0.0085). There were no differences in cell cycle status between CB and BM or PB. Our results suggest that the migrating potential of CB would be enhanced with increased CXCR4 expression on the NCs, but the adhesion potential of CB CD34+ cells would be less than that of PB and BM. These findings may help explain why the lower cell dose is required and engraftment is delayed in cord blood stem cell transplantation.

Keyword

Cell Adhesion Molecules; Nucleated Cells; Erythrocytes; Bone Marrow; Peripheral Blood; Fetal Blood

MeSH Terms

Antigens, CD34/metabolism
Antigens, CD44/*metabolism
Bone Marrow Cells/*metabolism
Cell Adhesion Molecules/*metabolism
Cell Cycle/*physiology
Cell Proliferation
Cell Separation
Fetal Blood/*cytology
Flow Cytometry
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells/*metabolism
Humans
Integrin alpha4/metabolism
Receptors, CXCR4/metabolism
Research Support, Non-U.S. Gov't

Figure

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  Fig. 1 Representative flow cytometric profile of CXCR4, CD44, CD49d and CD34 expression on the isolated nucleated cells from bone marrow (BM), mobilized peripheral blood (PB) and cord blood (CB).

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  Fig. 2 Representative cell cycle analysis of the isolated nucleated cells from bone marrow (BM), mobilized peripheral blood (PB) and cord blood (CB).

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