Ann Dermatol.  2012 Feb;24(1):94-98. 10.5021/ad.2012.24.1.94.

Effects of Murine Dermal Cells on the Regulation of Hair Growth Is Dependent on the Cell Number and Post-Natal Age of Newborn Mice

• Park KC
• Choi HR
• Na JI
• Cho HJ
• Nam KM
• Choi JW
• Na SY
• Huh CH

• KMID: 2156866
• DOI: http://doi.org/10.5021/ad.2012.24.1.94

Abstract

Dermal cells from neonatal mice can initiate the formation of hair follicles (HFs) when combined with adult mouse epidermal cells and transplanted subcutaneously into athymic mice. In the present study, the effects of dermal cells on HF formation were tested in terms of total cell number and the time course of cell harvest. Results demonstrated that the number of dermal cells is critical to the formation of HF. Furthermore, hair forming ability is rapidly decreasing as the neonatal mice age. To examine potential differences in gene expression, cDNA array was performed. Results demonstrate that numerous molecules which are directly involved in receptor and signaling correlated with decreased hair inductivity in early time points after delivery. It is reported that bone morphogenic protein (BMP)-6 and Wnt3a treatment increased hair inductivity of dermal papilla cells. But in our study, no changes were observed in the expression levels of BMP-6 and Wnt3a. However, several Wnt related genes demonstrate increased or decreased expression levels. Thus, our results suggest that co-ordinated regulation of these molecules will be important in hair neogenesis within our model system.

Keyword

Hair; Neonatal mouse

MeSH Terms

Adult
Animals
Bone Morphogenetic Protein 6
Cell Count
Gene Expression
Hair
Hair Follicle
Humans
Infant, Newborn
Mice
Mice, Nude
Oligonucleotide Array Sequence Analysis
Transplants
Bone Morphogenetic Protein 6

Figure

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  Fig. 1 Formation of nodules at inoculation sites according to different combination of dermal (105~106 cells) and epidermal cells (104~106 cells).

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  Fig. 2 Histological analysis of nodules according to different combination of dermal and epidermal cells (H&E, ×100).

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