J Vet Sci.  2014 Sep;15(3):423-426. 10.4142/jvs.2014.15.3.423.

A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C

Affiliations
  • 1State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. zhangjie03@caas.cn
  • 2Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China.
  • 3Lanzhou Jiaotong Univesity Bowen College, Lanzhou 730101, China.

Abstract

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription-PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.

Keyword

foot-and-mouth disease; foot-and-mouth disease virus serotype C; reverse transcription loop-mediated isothermal amplification; reverse transcription-PCR, sensitivity

MeSH Terms

Animals
Foot-and-Mouth Disease/*diagnosis
Foot-and-Mouth Disease Virus/genetics
Nucleic Acid Amplification Techniques/*methods/veterinary
Reverse Transcriptase Polymerase Chain Reaction/veterinary
Reverse Transcription/genetics
Sensitivity and Specificity
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