Exp Mol Med.  2014 Feb;46(2):e77. 10.1038/emm.2013.151.

Knockdown of 14-3-3zeta enhances radiosensitivity and radio-induced apoptosis in CD133+ liver cancer stem cells

• Lee YK
• Hur W
• Lee SW
• Hong SW
• Kim SW
• Choi JE
• Yoon SK

• KMID: 2155342
• DOI: http://doi.org/10.1038/emm.2013.151

Abstract

14-3-3zeta is related to many cancer survival cellular processes. In a previous study, we showed that silencing 14-3-3zeta decreases the resistance of hepatocellular carcinoma (HCC) to chemotherapy. In this study, we investigated whether silencing 14-3-3zeta affects the radioresistance of cancer stem-like cells (CSCs) in HCC. Knockdown of 14-3-3zeta decreased cell viability and the number of spheres by reducing radioresistance in CSCs after gamma-irradiation (IR). Furthermore, the levels of pro-apoptotic proteins were upregulated in CSCs via silencing 14-3-3zeta after IR. These results suggest that 14-3-3zeta knockdown enhances radio-induced apoptosis by reducing radioresistance in liver CSCs.

Keyword

14-3-3zeta; apoptosis; cancer stem-like cell; hepatocellular carcinoma; radioresistance

MeSH Terms

14-3-3 Proteins/genetics/*metabolism
Antigens, CD/genetics/*metabolism
Apoptosis Regulatory Proteins/genetics/metabolism
Carcinoma, Hepatocellular/genetics/metabolism
Cell Line, Tumor
*Gamma Rays
Glycoproteins/genetics/*metabolism
Humans
Liver Neoplasms/genetics/metabolism
Neoplastic Stem Cells/metabolism/*radiation effects
Peptides/genetics/*metabolism
*Radiation Tolerance
14-3-3 Proteins
Antigens, CD
Apoptosis Regulatory Proteins
Glycoproteins
Peptides