Cancer Res Treat.  2016 Jan;48(1):115-124. 10.4143/crt.2014.287.

Simultaneous Detection of Disseminated and Circulating Tumor Cells in Primary Breast Cancer Patients

Affiliations
  • 1Department of Obstetrics and Gynecology, University of Tuebingen, Tuebingen, Germany. andreas.hartkopf@med.uni-tuebingen.de
  • 2Department of Obstetrics and Gynecology, University of Heidelberg, Heidelberg, Germany.
  • 3Department of Obstetrics and Gynecology, University of Duesseldorf, Duesseldorf, Germany.

Abstract

PURPOSE
Disseminated tumor cells (DTCs) from bone marrow (BM) are a surrogate of minimal residual disease (MRD) in primary breast cancer (PBC) patients and associated with an adverse prognosis. However, BM sampling is an invasive procedure. Although there is growing evidence that circulating tumor cells (CTCs) from the blood are also suitable for monitoring MRD, data on the simultaneous detection of DTCs and CTCs are limited.
MATERIALS AND METHODS
We determined the presence of DTCs using immunocytochemistry and the pan-cytokeratin antibody A45-B/B3. CTCs were determined simultaneously using a reverse transcription-polymerase chain reaction-based assay (AdnaTest Breast Cancer) and CellSearch (at least one CTC per 7.5 mL blood). We compared the detection of DTCs and CTCs and evaluated their impact on disease-free and overall survival.
RESULTS
Of 585 patients, 131 (22%) were positive for DTCs; 19 of 202 (9%) and 18 of 383 (5%) patients were positive for CTCs, as shown by AdnaTest and CellSearch, respectively. No significant association was observed between DTCs and CTCs (p=0.248 and p=0.146 as shown by AdnaTest and CellSearch, respectively). The presence of DTCs (p=0.046) and the presence of CTCs as shown by CellSearch (p=0.007) were predictive of disease-free survival.
CONCLUSION
Our data confirm the prognostic relevance of DTCs and CTCs in patients with PBC. As we found no significant relationship between DTCs and CTCs, prospective trials should include their simultaneous detection. Within those trials, the question of whether or not DTCs and CTCs are independent subpopulations of malignant cell clones should be determined by molecular characterization.

Keyword

Circulating neoplastic cells; Bone marrow; Breast neoplasms; Residual neoplasm; Prognosis

MeSH Terms

Bone Marrow
Breast Neoplasms*
Breast*
Clone Cells
Disease-Free Survival
Humans
Immunohistochemistry
Neoplasm, Residual
Neoplastic Cells, Circulating*
Prognosis
Prospective Studies

Figure

  • Fig. 1. Impact of disseminated tumor cells (DTCs) from bone marrow on disease-free survival (A) and overall survival (B).

  • Fig. 2. Impact of circulating tumor cells (CTCs) from peripheral blood as determined by AdnaTest (A, B) and CellSearch (C, D) on disease-free survival (A, C) and overall survival (B, D).


Reference

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