Immune Netw.  2011 Dec;11(6):416-419. 10.4110/in.2011.11.6.416.

Th17 Responses Are Not Induced in Dextran Sodium Sulfate Model of Acute Colitis

Affiliations
  • 1Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju, Wonju 220-710, Korea. kjrhee@yonsei.ac.kr
  • 2Department of Biomedical Laboratory Science, Daegu Health College, Daegu 702-722, Korea.

Abstract

Dextran sodium sulfate (DSS) is a widely used chemical model for inflammatory bowel disease (IBD). It is thought that imbalances in the T helper (Th) cell subsets contribute to IBD. Recent studies suggest that the acute DSS-colitis model is polarized toward a Th1/Th17 profile based on RT-PCR analysis of colonic tissues. In the current study we determined whether colonic Th cells from DSS-colitis mice were skewed toward the Th17 profile. Mice were treated with 5% DSS for 7 days and colonic T cells isolated and examined for production of IFN-gamma (Th1 cell), IL-4 (Th2 cell) and IL-17 (Th17 cell) by intracellular flow cytometry. We found that the percentage of colonic Th17 cells were similar to non-treated controls but the percentage of Th1 cells were elevated in DSS-colitis mice. These results suggest that in the acute DSS-colitis model the colonic Th cells exhibit a Th1 profile and not a Th17 profile.

Keyword

Dextran sodium sulfate; Inflammatory bowel disease; Th17 cell; Colitis

MeSH Terms

Animals
Colitis
Colon
Dextrans
Flow Cytometry
Inflammatory Bowel Diseases
Interleukin-17
Interleukin-4
Mice
Models, Chemical
Sodium
Sulfates
T-Lymphocytes
Th1 Cells
Th17 Cells
Dextrans
Interleukin-17
Interleukin-4
Sodium
Sulfates
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