Korean J Clin Microbiol.  2002 Mar;5(1):52-58.

Diagnosis of Parasitic Infection by ELISA test

• Choi TY
• Ahn MH
• Ha SE
• Choi HK
• Ryu JS

Abstract

BACKGROUND: ELISA test is commonly used for diagnosis of parasite infection. This experiment was performed for detecting positive sera against Clonorchis sinensis, Paragonimus westermani, cysticercus, sparganum, Aisakis larvae, Toxoplasma gondii and Trichomonas vaginalis in patient's sera with ELISA and Western blot analysis.
METHODS
Two hundred sera were collected from clinical laboratory of Hanyang University Hospital(Seoul, Kuri). Antigens of parasites were prepared from rabbit (C. sinensis), dog (P. westermani), hog (cysticercus, from Yonsei University), patient (sparganum), mackerel (Anisakis larvae), mouse (T. gondii), cultivation in Trypticase-Yeast extract-Maltose medium (T. vaginalis). ELISA and Western blot was conducted with several parasite antigens and patient's sera.
RESULTS
Positive antibody titers of P. westermani, Anisakis, C. sinensis were observed 12.7%, 11.0%, and 7.0% of patient's sera, respectively. Nineteen sera among 200 patients showed cross reactions with other parasites. On Western blot, there were several antigenic bands with patient's sera, i.e., 3/5 sera of C. sinensis, 2/2 sera of P. westermani, 1/4 sera of sparganum, and 0/4 sera of cysticercus. COCLUSIONS: ELISA is a convenient method for detecting parasite infections. But purification of antigens is necessary and Western blot analysis may reduce the false positive reactions of infection.

Keyword

Clonorchis sinensis; Paragonimus westermani; cysticercus; spargenum; Anisakis; Toxoplasma gondii; Trichomonas vaginalis; ELISA; Western blot

MeSH Terms

Animals
Anisakis
Blotting, Western
Clonorchis sinensis
Cross Reactions
Cysticercus
Diagnosis*
Dogs
Enzyme-Linked Immunosorbent Assay*
False Positive Reactions
Humans
Larva
Mice
Paragonimus westermani
Parasites
Perciformes
Sparganum
Toxoplasma
Trichomonas vaginalis