Korean J Clin Pathol.  1997 Dec;17(6):1176-1181.

Detection of a Familial Y/l5 Translocation by FISH, G-Banding, C-Banding, and Ag-NOR Stain

• Lee KM
• Kim KH
• Han JY
• Je GH
• Shaffer LG

Abstract

Prenatal chromosome analysis of amniotic cells at 18 weeks of gestation showed a male fetus to carry a large 15p+ derivative chromosome inherited from his mother. Extra genetic material on the short arm of chromosome IS was silver-negative with Ag-NOR (nucleolus organizer regions) stain, but stained darkly with C-banding method like the distal heterochromatic segment of the Y long arm. Fluorescence in situ hybridization (FISH) using two DNA probes (DYZ1 and D15Zl) showed a red fluorescent signal on 15p+ In addition to a green chromosome 15 centromere signal, confirming 15p to be from the distal Yq heterochromatin.

MeSH Terms

Arm
Centromere
Chromosomes, Human, Pair 15
DNA Probes
Fetus
Fluorescence
Heterochromatin
Humans
In Situ Hybridization
Male
Mothers
Pregnancy
DNA Probes
Heterochromatin