Korean J Thorac Cardiovasc Surg.  2009 Feb;42(1):1-8.

The Effects of Moxifloxacin in Endotoxin-induced Acute Lung Injury

• Baek JH
• Lee JH
• Lee JC
• Lee DH
• Moon YS
• Lee YM

Abstract

BACKGROUND: The pathophysiology of acute respiratory distress syndrome with sepsis is acute lung injury (ALI) that's' caused by endotoxin (LPS). We evaluate effects of moxifloxacin on LPS-induced ALI in a rat model. MATERIAL AND METHOD: The rats were divided into 3 groups as the control group (C), the LPS insult group (L), and the LPS+moxifloxacin treated group (L-M). ALI was induced by endotracheal instillation of E.coli LPS, then moxifloxacin was given in 30 minutes. Five hours later, we checked the lung weight/body weight ratio(the L/BW ratio), the protein & neutrophils in the bronchoalveolar lavage fluid (BALF), the myeloperoxidase (MPO) activity & the malondialdehyde (MDA) content, the expressions of cytosolic and secretory phospholipase A2 (c, sPLA2), and the morphology of the lung with using a light microscope. RESULT: The L/BW ratio, the protein content and the neutrophil count in the BALF, and the MPO activity and the MDA content in lung were significantly increased in group L compared to group C, and these factors were markedly decreased in group L-M compare to group L. The cPLA2 expression and the sPLA2 expression were increased in group L and the cPLA2 expression was decreased in group L-M. Yet the sPLA2 expression was not changed in group L-M. Morphologically, many inflammatory findings were observed in group L, but not in group L-M.
CONCLUSION
Many of the inflammatory changes of ALI that were caused by LPS insult were ameliorated by moxifloxacin treatment.

Keyword

Acute respiratory distress syndrome; Neutrophils; Phospholipase A

MeSH Terms

Acute Lung Injury
Animals
Aza Compounds
Bronchoalveolar Lavage Fluid
Cytosol
Light
Lung
Malondialdehyde
Neutrophils
Peroxidase
Phospholipases A2, Secretory
Quinolines
Rats
Respiratory Distress Syndrome, Adult
Sepsis
Aza Compounds
Malondialdehyde
Peroxidase
Phospholipases A2, Secretory
Quinolines