Korean J Blood Transfus.  2015 Apr;26(1):47-53. 10.17945/kjbt.2015.26.1.47.

Performance Evaluation of Unexpected Antibody Screening Test Including Di(a) Cell Using 3% Surgiscreen Sub-Code D in Korean Multi-Transfused Patients

Affiliations
  • 1Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Korea. hhkim@pusan.ac.kr

Abstract

BACKGROUND
The Dia antigen has been found to have a relatively higher incidence among Korean populations. However, the current popular antibody screening panels contain no Dia positive cells. To prevent hemolytic transfusion reaction, screening for unexpected antibody plus screening for Dia positive cells should be performed. In this study, we evaluate the performance of the 3% Surgiscreen Sub-code D (Ortho-Clinical Diagnostics, USA) manufactured as a 3-cell panel including Dia cell versus the ID-DiaCell I-II (DiaMed, Switzerland) as a 2-cell panel plus ID-DiaCell Dia+ (DiaMed, Switzerland) in screening for irregular red blood cell alloantibodies.
METHODS
From December 13, 2013 to April 24, 2014, we tested the 3% Surgiscreen by the AutoVue Innova system and the ID-DiaCell in parallel to evaluate reagent sensitivity in detecting irregular antibodies in multi-transfused patients' plasma or serum. Identification of unexpected antibody tests was performed for positive screening results.
RESULTS
Antibody-positive rates were 4.2% (79/1885) and 4.6% (87/1885) for antibody screening with the 3% Surgiscreen and the ID-DiaCell, respectively. Among the 1885 samples, 1875 (99.5%) showed concordant results between the 2 methods, while 10 results differed. From the 10 discrepancies, 1 result was positive only on the 3% Surgiscreen. The prevalence of anti-Dia antibody was 10.1% and 9.2% in the 3% Surgiscreen and the ID-DiaCell, respectively.
CONCLUSION
The 3% Surgiscreen manufactured as 3-cell showed a high concordance rate ompared to standard methods. The prevalence of anti-Dia showed no difference between the 2 reagents.

Keyword

Dia antigen; anti-Di(a); 3% Surgiscreen; Unexpected antibody screening test; Multiple transfused patients

MeSH Terms

Antibodies
Blood Group Incompatibility
Erythrocytes
Humans
Incidence
Indicators and Reagents
Isoantibodies
Mass Screening*
Plasma
Prevalence
Antibodies
Indicators and Reagents
Isoantibodies
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