Nutr Res Pract.  2008 Dec;2(4):240-246. 10.4162/nrp.2008.2.4.240.

Effect of butanol fraction from Cassia tora L. seeds on glycemic control and insulin secretion in diabetic rats

• Nam J
• Choi H

• KMID: 2139198
• DOI: http://doi.org/10.4162/nrp.2008.2.4.240

Abstract

Cassia tora L. seeds have previously been reported to reduce blood glucose level in human and animals with diabetes. In the present study, the effects of Cassia tora L. seed butanol fraction (CATO) were studied on postprandial glucose control and insulin secretion from the pancreas of the normal and diabetic rats. Diabetes was induced by an i.p. injection of Streptozotocin (55 mg/kg BW) into the male Sprague-Dawley rats. The postprandial glucose control was monitored during a 240 min-period using a maltose loading test. In normal rats, rats fed CATO (20 mg/100 g BW/d) showed lower postprandial glucose levels in all the levels from 30 min up to 180 min than those in the control rats without CATO (p<0.05). In diabetic rats, those levels in the CATO group seemed to be lower during the 30~180 min, but only glucose level at 30 min showed significant difference compared to that in the control group. Moreover, CATO delayed the peak time of the glucose rise in both normal and diabetic rats in the glucose curves. On the other hand, when CATO was administered orally to the diabetic rats for 5 days, 12 hr fasting serum glucose level was decreased in the diabetic rats (p<0.05). Degree of a decrease in 12 hr fasting serum insulin levels was significantly less in the diabetic CATO rats as compared to diabetic control rats. On the last day of feeding, beta cells of the pancreas were stimulated by 200 mg/dL glucose through a 40 min-pancreas perfusion. Amounts of the insulin secreted from the pancreas during the first phase (11~20 min) and the second phase (21~40 min) in the CATO fed diabetic rats were significantly greater than those in the diabetic control group (p<0.05). These findings indicated that constituents of Cassia tora L. seeds have beneficial effect on postprandial blood glucose control which may be partially mediated by stimulated insulin secretion from the pancreas of the diabetic rats.

Keyword

Diabetes; Cassia tora L. seeds butanol fraction; pancreas perfusion; insulin secretion

MeSH Terms

Animals
Blood Glucose
Cassia
Fasting
Glucose
Hand
Humans
Insulin
Male
Maltose
Pancreas
Perfusion
Rats
Rats, Sprague-Dawley
Seeds
Streptozocin
Triazines
Blood Glucose
Glucose
Insulin
Maltose
Streptozocin
Triazines

Figure

• 

  Fig. 1 Fasting serum glucose levels in normal and diabetic rats administered Cassia tora L. butanol fraction for 5 days. Values are mean ± SE. Number of rats is 6 per each group. Bars with different letters are significantly different by ANOVA and Scheffé test at p<0.05. CONT: Rats were fed on pellet diet and water for 5 days. CATO: Rats were fed on pellet diet and administered Cassia tora. L. butanol fraction (20 mg/100 g BW/d) for 5 days.

• 

  Fig. 2 Insulin amounts secreted from the pancreas of the normal rats administered Cassia tora L. butanol fraction for 5 days. Values are mean ± SE. Number of rats is 6 per each group. There were no significant differences between the CONT and CATO by Student's t-test. 0-10 min: perfusion period of no glucose stimulation. 11-20 and 21-40 min: perfusion period of 200 mg/dL glucose stimulation. CONT: Rats were fed on pellet diet and water for 5 days. CATO: Rats were fed on pellet diet and administered Cassia tora. L. butanol fraction (20 mg/100 g BW/d)) for 5 days.

• 

  Fig. 3 Insulin amounts secreted from the pancreas of the diabetic rats administered Cassia tora L. butanol extract for 5 days. Values are mean ± SE. Number of rats is 6 per each group. Bars with an asterisk are significantly different compared to the value of CONT by Student's t-test at p<0.05. 0-10 min: perfusion period of no glucose stimulation. 11-20 and 21-40 min: perfusion period of 200 mg/dL glucose stimulation. CONT: Rats were fed on pellet diet and water for 5 days. CATO: Rats were fed on pellet diet and administered Cassia tora. L. butanol fraction (20 mg/100 g BW/d) for 5 days.

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