J Korean Neurol Assoc.
2002 Nov;20(6):668-681.
Poly (ADP-Ribose) Polymerase immunoreactivity in Motor Neurons and Astrocytes in the Spinal Cord of Sporadic Amyotrophic Lateral Sclerosis Patients
- Affiliations
-
- 1Department of Neurology, Pathology, College of Medicine, Hanyang University, Seoul, Korea. kimsh1@hanyang.ac.kr
- 2Department of Neurology, Baylor College of Medicine, Houston, Texas, U.S.A.
- 3Department of Neurology, University of Szeged, Hungary.
- 4Department of Biophysics, Biological Research Center, Szeged, Hungary.
Abstract
-
BACKGROUND: The evidence for increased oxidative stress and DNA damage in amyotrophic lateral sclerosis (ALS) prompted studies to determine if the expression of poly (ADP-ribose) polymerase (PARP) is increased in ALS.
METHOD: Twenty Spinal cord specimens were obtained at the autopsy of sALS patients (n=11) and age-matched controls with non-neurological diseases (n=9).
RESULTS
Using western analyses of postmortem tissue, we demonstrated that PARP-immunoreactivity (PARP-IR) was increased three-fold in spinal cord tissues of sporadic ALS (sALS) patients compared with non-neurological disease controls. Despite the increased PARP-IR, PARP mRNA expression was not increased significantly. Immunohistochemical analyses revealed PARP-IR was increased in both white and gray matter of sALS spinal cord. While PARP-IR was predominantly seen in astrocytes, large motor neurons displayed reduced staining compared with the controls. PARP-IR was increased in the pellet fraction of sALS homogenates compared with the control homogenates, representing potential PARP binding to chromatin or membranes and suggesting a possible mechanism of PARP stabilization.
CONCLUSIONS
The present results demonstrate glial alterations in sALS tissue and support the role of glial alterations in sALS pathogenesis.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Increased Neuronal and Glial Poly (ADP-Ribose) Polymerase Immunoreactivity in the Brain of Sporadic Amyotrophic Lateral Sclerosis
- Impaired Nucleoporins Are Present in Sporadic Amyotrophic Lateral Sclerosis Motor Neurons that Exhibit Mislocalization of the 43-kDa TAR DNA-Binding Protein
- Spinal anesthetic management for discectomy in a patient with amyotrophic lateral sclerosis: A case report
- Evolving Diagnostic Criteria in Amyotrophic Lateral Sclerosis and Its Differential Diagnosis
- Prolyl Isomerase Pin1 Expression in the Spinal Motor Neurons of Patients With Sporadic Amyotrophic Lateral Sclerosis
