J Korean Assoc Oral Maxillofac Surg.  2002 Jun;28(3):169-174.

Production Of Gm-Csf And Tgf-beta 1 In Irradiated Human Gingival Fibroblasts Cultured With Lipopolysaccharide

  • 1Department of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University, Korea. omslee@ewha.ac.kr
  • 2Department of Dentistry, College of Medicine, Ewha Womans University, Korea.
  • 3Department of Microbiology, College of Medicine, Kosin University, Korea.


PURPOSE: Irradiation in the oral cancer patients causes early and late complications such as intraoral mucositis and fibrosis, with a various expression of GM-CSF and TGF-beta. The purpose of this study was to investigate the production of GM-CSF and TGF-beta1 by the irradiated human gingival fibroblasts cultivated with lipopolysaccharide.
Irradiated (total dose, 60 Gy) human gingival fibroblasts were incubated with LPS. Culture supernatants that were collected at 24, 48, and 72 hours were assessed for GM-CSF and TGF-beta1 by enzyme-linked immunosorbent assay.
1. GM-CSF production in nomal gingival fibroblasts was increased with incubation time, but decreased with incubation time in irradiated gingival fibroblasts. GM-CSF production in both normal and irradiated gingival fibroblasts induced with LPS was higher than the control. 2. TGF-beta1 production in normal gingival fibroblasts was decreased after 24 hours, but, it was increased until 48 hours in irradiated gingival fibroblasts. TGF-beta1 production in normal gingival fibroblasts exposed with LPS was higher than the control. Conversely, It was lower than the control in irradiated gingival fibroblasts exposed with LPS.
This indicates that irradiation in gingival fibroblasts may play an important role in radiation-induced intraoral mucositis and fibrosis. However, LPS decreases the production of TGF-beta1 in the irradiated gingival fibroblasts.


Gingival fibroblasts; Irradiation; lipopolysaccharide
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