Gut Liver.  2013 Mar;7(2):221-227.

Lipoic Acid Prevents the Changes of Intracellular Lipid Partitioning by Free Fatty Acid

Affiliations
  • 1Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea. noshin1004@google.co.kr
  • 2Department of Occupational and Environmental Medicine, Soonchunhyang University College of Medicine, Cheonan, Korea.
  • 3Department of Radiology, Hanyang University College of Medicine, Seoul, Korea.
  • 4Department of Pathology, Eulji University School of Medicine, Seoul, Korea.

Abstract

BACKGROUND/AIMS
It is suggested that the hepatic lipid composition is more important than lipid quantity in the pathogenesis of non-alcoholic steatohepatitis. We examined whether lipoic acid (LA) could alter intrahepatic lipid composition and free cholesterol distribution.
METHODS
HepG2 cells were cultured with palmitic acid (PA) with and without LA. Apoptosis, changes of the mitochondrial structure, intracellular lipid partitioning, and reactive oxygen species (ROS) activity were measured.
RESULTS
Free fatty acid (FA) increased apoptosis, and LA co-treatment prevented this lipotoxicity (apoptosis in controls vs PA vs PA+LA, 0.5% vs 19.5% vs 1.6%, p<0.05). LA also restored the intracellular mitochondrial DNA copy number (553+/-33.8 copies vs 291+/-14.55 copies vs 421+/-21.05 copies, p<0.05) and reversed the morphological changes induced by PA. In addition, ROS was increased in response to PA and was decreased in response to LA co-treatment (41,382 relative fluorescence unit [RFU] vs 43,646 RFU vs 41,935 RFU, p<0.05). LA co-treatment increased the monounsaturated and polyunsaturated FA concentrations and decreased the total saturated FA fraction. It also prevented the movement of intracellular free cholesterol from the cell membrane to the cytoplasm.
CONCLUSIONS
LA opposes free FA-generated lipotoxicity by altering the intracellular lipid composition and free cholesterol distribution.

Keyword

Thioctic acid; Lipotoxicity; Lipid partitioning; Non-alcoholic steatohepatitis; Liver cirrhosis

MeSH Terms

Apoptosis
Cell Membrane
Cholesterol
Coat Protein Complex I
DNA, Mitochondrial
Fatty Liver
Fluorescence
Hep G2 Cells
Liver Cirrhosis
Palmitic Acid
Reactive Oxygen Species
Thioctic Acid
Cholesterol
Coat Protein Complex I
DNA, Mitochondrial
Palmitic Acid
Reactive Oxygen Species
Thioctic Acid
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