J Vet Sci.  2015 Dec;16(4):483-489. 10.4142/jvs.2015.16.4.483.

Profiling of antimicrobial resistance and plasmid replicon types in beta-lactamase producing Escherichia coli isolated from Korean beef cattle

• Shin SW
• Jung M
• Shin MK
• Yoo HS

• KMID: 2133631
• DOI: http://doi.org/10.4142/jvs.2015.16.4.483

Abstract

In this study, 78 isolates of Escherichia coli isolated from Korean beef cattle farms were investigated for the production of extended-spectrum beta-lactamase (ESBL) and/or AmpC beta-lactamase. In the disc diffusion test with ampicillin, amoxicillin, cephalothin, ceftiofur, cefotaxime, ceftazidime, and cefoxitin, 38.5% of the isolates showed resistance to all of ampicillin, amoxicillin, and cephalothin. The double disc synergy method revealed that none of the isolates produced ESBL or AmpC beta-lactamases. DNA sequencing showed that all isolates encoded genes for TEM-1-type beta-lactamase. Moreover, 78.2% of the isolates transferred the TEM-1-type beta-lactamase gene via conjugation. In plasmid replicon typing of all donors, IncFIB and IncFIA were identified in 71.4% and 41.0% of plasmids, respectively. In transconjugants, IncFIB and IncFIA were the most frequent types detected (61.5% and 41.0%, respectively). Overall, the present study indicates that selection pressures of antimicrobials on beta-lactamases in beef cattle may be low relative to other livestock animals in Korea. Moreover, to reduce selection pressure and dissemination of beta-lactamase, the long-term surveillance of antimicrobial use in domestic beef cattle should be established.

Keyword

beta-lactamase; antimicrobial resistance; Escherichia coli; plasmid replicon typing

MeSH Terms

Amoxicillin
Ampicillin
Animals
beta-Lactamases*
Cattle*
Cefotaxime
Cefoxitin
Ceftazidime
Cephalothin
Diffusion
Escherichia coli*
Escherichia*
Humans
Korea
Livestock
Plasmids*
Replicon*
Sequence Analysis, DNA
Tissue Donors
Amoxicillin
Ampicillin
Cefotaxime
Cefoxitin
Ceftazidime
Cephalothin
beta-Lactamases

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