J Vet Sci.  2015 Dec;16(4):475-481. 10.4142/jvs.2015.16.4.475.

Helicobacter apodemus sp. nov., a new Helicobacter species identified from the gastrointestinal tract of striped field mice in Korea

Affiliations
  • 1Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, BIO-MAX Institute, Program for Cancer Biology, and Interdisciplinary Program for Bioinformatics, BK21Plus Program for Creative Veterinary Science Research, Research Institut
  • 2Incheon International Airport Imported Food Inspection Center, Gyeongin Regional Food and Drug Administration, Ministry of Food and Drug Safety, Incheon 22382, Korea.
  • 3Laboratory of Veterinary Public Health, College of Veterinary Medicine, BIO-MAX Institute, Program for Cancer Biology, and Interdisciplinary Program for Bioinformatics, BK21Plus Program for Creative Veterinary Science Research, Research Institute for Vete
  • 4College of Pharmacy, Gachon University, Incheon 21936, Korea.
  • 5College of Veterinary Medicine, Jeju National University, Jeju 63241, Korea.
  • 6College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
  • 7Department of Medical Genetics, College of Medicine, Hallym University, Chuncheon 24252, Korea.
  • 8Biomedical Mouse Resource Center, Korea Research Institute for Bioscience and Biotechnology, Ochang 34141, Korea.

Abstract

A novel Helicobacter species was identified from the gastrointestinal tract of the Korean striped field mouse (Apodemus agrarius). Biochemical testing, ultrastructure characterization, and 16S rRNA gene sequence analysis suggested that this bacterium represents a distinct taxon. The bacterium was positive for urease activity, susceptible to cephalothin and nalidixic acid, and weakly positive for oxidase and catalase activity. Electron microscopy revealed that the bacterium has spirally curved rod morphology with singular bipolar nonsheathed flagella. Genotypically, the isolated bacterial strains (YMRC 000215, YMRC 000216, and YMRC 000419) were most closely related to a reference strain of Helicobacter mesocricetorum (97.25%, 97.32%, and 97.03% 16S rRNA sequence similarities, respectively). The 16S rRNA sequences of these strains were deposited into GenBank under accession numbers AF284754, AY009129, and AY009130, respectively. We propose the name Helicobacter apodemus for this novel species.

Keyword

16S rRNA; Apodemus agrarius; Helicobacter apodemus; striped field mouse

MeSH Terms

Animals
Catalase
Cephalothin
Databases, Nucleic Acid
Flagella
Gastrointestinal Tract*
Genes, rRNA
Helicobacter*
Korea*
Mice*
Microscopy, Electron
Murinae*
Nalidixic Acid
Oxidoreductases
Sequence Analysis
Urease
Catalase
Cephalothin
Nalidixic Acid
Oxidoreductases
Urease

Figure

  • Fig. 1 Transmission electron micrograph of a negatively stained Helicobacter sp. Nov. cell isolated from the cecum of Apodemus agrarius. (A) YMRC 000215. (B) YMRC 000215. Scale bars = 1 µm (A), or 0.2 µm (B).

  • Fig. 2 Phylogenetic tree of strains isolated in this study and members of other genera generated using the neighbor-joining method.


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