J Vet Sci.  2015 Dec;16(4):423-429. 10.4142/jvs.2015.16.4.423.

Elevated level of renal xanthine oxidase mRNA transcription after nephropathogenic infectious bronchitis virus infection in growing layers

• Lin H
• Huang Q
• Guo X
• Liu P
• Liu W
• Zou Y
• Zhu S
• Deng G
• Kuang J
• Zhang C
• Cao H
• Hu G

• KMID: 2133624
• DOI: http://doi.org/10.4142/jvs.2015.16.4.423

Abstract

To assess relationships between xanthine oxidase (XOD) and nephropathogenic infectious bronchitis virus (NIBV) infection, 240 growing layers (35 days old) were randomly divided into two groups (infected and control) of 120 chickens each. Each chicken in the control and infected group was intranasally inoculated with 0.2 mL sterile physiological saline and virus, respectively, after which serum antioxidant parameters and renal XOD mRNA expression in growing layers were evaluated at 8, 15 and 22 days post-inoculation (dpi). The results showed that serum glutathione peroxidase and superoxide dismutase activities in the infected group were significantly lower than in the control group at 8 and 15 dpi (p < 0.01), while serum malondialdehyde concentrations were significantly higher (p < 0.01). The serum uric acid was significantly higher than that of the control group at 15 dpi (p < 0.01). In addition, the kidney mRNA transcript level and serum activity of XOD in the infected group was significantly higher than that of the control group at 8, 15 and 22 dpi (p < 0.05). The results indicated that NIBV infection could cause the increases of renal XOD gene transcription and serum XOD activity, leading to hyperuricemia and reduction of antioxidants in the body.

Keyword

antioxidant; growing layers; hyperuricemia; nephropathogenic infectious bronchitis virus; xanthine oxidase

MeSH Terms

Antioxidants
Chickens
Glutathione Peroxidase
Hyperuricemia
Infectious bronchitis virus*
Kidney
Malondialdehyde
RNA, Messenger*
Superoxide Dismutase
Uric Acid
Xanthine Oxidase*
Xanthine*
Antioxidants
Glutathione Peroxidase
Malondialdehyde
RNA, Messenger
Superoxide Dismutase
Uric Acid
Xanthine
Xanthine Oxidase

Figure

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  Fig. 1 Serum xanthine oxidase (XOD) activities in chickens in the control and infected groups. N = 12 per group at each time. Significant differences are indicated by *p < 0.05 and **p < 0.01 in comparison with control group.

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  Fig. 2 Serum glutathione peroxidase (GSH-PX) activities in chickens in the control and infected groups. N = 12 per group at each time. Significant differences are indicated by **p < 0.01 in comparison with control group.

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  Fig. 3 Serum superoxide dismutases (SOD) activities in chickens in the control and infected groups. N = 12 per group at each time. Significant differences are indicated by **p < 0.01 in comparison with control group.

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  Fig. 4 Serum uric acid (UA) contents in chickens in the control and infected groups. N = 12 per group at each time. Significant differences are indicated by **p < 0.01 in comparison with control group.

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  Fig. 5 Serum malondialdehyde (MDA) contents in chickens in the control and infected groups. N = 12 per group at each time. Significant differences are indicated by **p < 0.01 in comparison with control group.

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  Fig. 6 Levels of renal XOD mRNA transcript in chickens in the control and infected groups. N = 12 per group at each time. Significant differences are indicated by **p < 0.01 in comparison with control group.

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