J Rhinol.  2005 Nov;12(2):88-91.

Effects of Peroxynitrite on HSP 70 Expression in Cultured Normal Human Nasal Epithelial Cell

• Jang TY
• Choi YS
• Kim KS
• Ko KJ
• Kim TM

Abstract

BACKGROUND AND OBJECTIVES: Nitric Oxide (NO) is an endogenous mediator first characterized as an endothelium-derived relaxing factor. It is now recognized as a key mediator in many physiological process such as vasodilatation, neurotransmission, host defense, and iron metabolism. However, much remains to be determined about the pathophysiological role of NO in the airway. Peroxynitrite, which is synthesized by NO, is the diret cause of cellular toxicity in inflammatory reaction. In this study, we investigated the cellular toxcity of peroxynitrite by the expression of Heat-shock proteins 70 (HSP 70) in normal human nasal epithelium (NHNE) at the inflammatory conditions
MATERIALS AND METHODS
3-Morpholinosydronimone clorhydrate which is a peroxynitrite donor was mixed in the media of cultured NHNE cell.
RESULTS
HSP 70 was expressed at the peroxynitrite environment of cultured NHNE cells and HSP 70 mRNA was detected with a time-dependent increasing pattern.
CONCLUSION
Peroxynitrite may have a cytotoxic effect, and inhibition of peroxynitrite synthesis may have an important role for controlling the cytotoxic and inflammatory conditions of rhinitis and sinusitis.

Keyword

Nitric oxide; Peroxynitrite; Heat-shock proteins 70

MeSH Terms

Endothelium-Dependent Relaxing Factors
Epithelial Cells*
HSP70 Heat-Shock Proteins
Humans*
Iron
Metabolism
Nasal Mucosa
Nitric Oxide
Peroxynitrous Acid*
Physiological Processes
Rhinitis
RNA, Messenger
Sinusitis
Synaptic Transmission
Tissue Donors
Vasodilation
Endothelium-Dependent Relaxing Factors
HSP70 Heat-Shock Proteins
Iron
Nitric Oxide
Peroxynitrous Acid
RNA, Messenger