Allergy Asthma Immunol Res.  2015 Nov;7(6):599-604. 10.4168/aair.2015.7.6.599.

Attenuated Allergenic Activity of Ovomucoid After Electrolysis

• Kido J
• Matsumoto

• KMID: 2130267
• DOI: http://doi.org/10.4168/aair.2015.7.6.599

Abstract

Ovomucoid (OMC) is the most prominent allergen causing hen's egg allergy, containing disulfide (S-S) bonds that may be responsible for its allergic action. As S-S bonds may be reduced during electrolysis, this study was undertaken to evaluate modulation of the allergic action of OMC after electrolysis. Electrolysis was carried out for 1% OMC containing 1% sodium chloride for 30 minutes with a voltage difference of 90 V, 0.23 A (30 mA/cm2). Protein assays, amino acid measurement, and mass spectrometry in untreated OMC and OMC on both the anode and cathode sides after electrolysis were performed. Moreover, 21 patients with IgE-mediated hen's egg allergy were evaluated by using the skin prick test (SPT) for untreated OMC and OMC after electrolysis. The allergic action of OMC was reduced after electrolysis on both the anode and cathode sides when evaluated by the SPT. The modifications of OMC on electrolysis caused the loss of 2 distinct peptide fragments (57E-63K and 123H-128R) as seen on matrix-associated laser desorption/ionization time-of-flight mass spectrometry. The total free SH groups in OMC were increased on the cathode side. Although the regions of S-S broken bonds were not determined in this study, the change in S-S bonds in OMC on both the anode and cathode sides may reduce the allergenic activity.

Keyword

Electrolysis; hen's egg allergy; ovomucoid

MeSH Terms

Egg Hypersensitivity
Electrodes
Electrolysis*
Humans
Mass Spectrometry
Ovomucin*
Peptide Fragments
Skin
Sodium Chloride
Ovomucin
Peptide Fragments
Sodium Chloride

Figure

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  Fig. 1 Pictures of the electrolysis apparatus. (A) Whole image of electrolysis apparatus. (B) Magnified image of the surrounding platinum electrode plates. (C) Cooling system for electrolysis apparatus.

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  Fig. 2 Representative results of skin prick tests.

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  Fig. 3 MALDI-ToF-MS SPECTRA of uOMC (upper), cOMC (middle), and aOMC (lower) after tryptic digestion.

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  Fig. 4 2D-PAGE of uOMC (upper), cOMC (middle), and aOMC (lower).

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