J Korean Ophthalmol Soc.  2000 May;41(5):1054-1061.

The Minimal Inhibitory Concentrations of Antifungal Agents for Clinical Isolates in Fungal Keratitis

• Sohn SW
• Hahn YH

Abstract

This study was performed for the standardization and proper selection of effective antifungal agents by measuring the minimal inhibitory concentra-tions[MICs]of antifungals to fungi, separated from keratitis patients.Two strains of A.fumigatus and single strain of F.solani, A.falciforme, and A . alternata were used for this test.Standard powders of miconazole, itraconazole, clotrimazole, ketoconazole, and amphotericin B were used as antifungal agents. Microscopic and macroscopic measurements of MIC after 24, 48 and 72 hours of inoculation[105 conidia /ml]into YNB broth with culture temperature of 25 degrees C were performed by use of broth microdilution method.The results are as follows : itraconazole, amphotericin B, and clotrimazole were effective against A.fumigatus.F.solani showed resistance to all kinds of antifungal agents.A.falciforme and A.alternata were sensitive to amphotericin B and itraconazole, respectively. Further studies may be needed for the standardized measurement of MIC against filamentous fungi and also for in Vitro-in Vivo correlations for the treatment of fungal keratitis.

Keyword

Antifungal agent; Fungal keratitis; Microscopic minimal inhibitory concentration[MIC]; Susceptibility test

MeSH Terms

Amphotericin B
Antifungal Agents*
Clotrimazole
Fungi
Itraconazole
Keratitis*
Ketoconazole
Miconazole
Powders
Spores, Fungal
Amphotericin B
Antifungal Agents
Clotrimazole
Itraconazole
Ketoconazole
Miconazole
Powders