The Topical Effects of Antibiotics and Aprotinin in Experimental Pseudomonas Ketaititis

Hong, Nae Sun; Kim, Jae Chan; Shyn, Kyuog Hwan

Abstract

The effects of the topically applied antibiotics and Aprotinin were evaluated in the experimentally induced pseudomonas aeruginosa keratitis in rabbits. 40 albino rabbits were used for this study and were divided into 8 groups according to the treatment protocols as follows: Aprotinin 40u/ml, 1,OOOu/ml, 1O,000u/ml, antibiotics (Ciprofloxacin and Tobramycin), and antibiotics and Aprotinin combined-treated group. The drugs were instilled in the rabbits, cornea 6 times per day for 2 weeks. The clinical evaluation was performed using a hand-held slit lamp every day. For the histopathologic examination, we enucleated every samples at different time intervals and prepared specimens for light microscope. The results were Aprotinin-treated group showed progression of ulcer infiltrates regardless of its concentrations. There was a statistically significant difference between the antibiotics-treated group and the control group (p<0.05). However, the combination of Aprotinin and antibiotics were not able to gain statistical significance when compare with antibiotics alone (p>0.05). There was no clinical and histopathological differences in the rabbit cornea between ciprofloxacin and tobramycin treated group (p>0.05).