Arch Plast Surg.
2012 Nov;39(6):593-599. 10.5999/aps.2012.39.6.593.
Possibility of Undifferentiated Human Thigh Adipose Stem Cells Differentiating into Functional Hepatocytes
- Affiliations
-
- 1Department of Plastic and Reconstructive Surgery, Eulji General Hospital, Eulji University College of Medicine, Seoul, Korea. joaljh@eulji.ac.kr
- 2Eulji Medi-Bio Research Institute, Eulji University, Seoul, Korea.
- 3Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
- 4CL Aesthetic Clinic, Seoul, Korea.
- 5Department of Biotechnology, Seoul Women's University, Seoul, Korea.
- KMID: 2120708
- DOI: http://doi.org/10.5999/aps.2012.39.6.593
Abstract
- BACKGROUND
This study aimed to investigate the possibility of isolating mesenchymal stem cells (MSCs) from human thigh adipose tissue and the ability of human thigh adipose stem cells (HTASCs) to differentiate into hepatocytes.
METHODS
The adipose-derived stem cells (ADSCs) were isolated from thigh adipose tissue. Growth factors, cytokines, and hormones were added to the collagen coated dishes to induce the undifferentiated HTASCs to differentiate into hepatocyte-like cells. To confirm the experimental results, the expression of hepatocyte-specific markers on undifferentiated and differentiated HTASCs was analyzed using reverse transcription polymerase chain reaction and immunocytochemical staining. Differentiation efficiency was evaluated using functional tests such as periodic acid schiff (PAS) staining and detection of the albumin secretion level using enzyme-linked immunosorbent assay (ELISA).
RESULTS
The majority of the undifferentiated HTASCs were changed into a more polygonal shape showing tight interactions between the cells. The differentiated HTASCs up-regulated mRNA of hepatocyte markers. Immunocytochemical analysis showed that they were intensely stained with anti-albumin antibody compared with undifferentiated HTASCs. PAS staining showed that HTASCs submitted to the hepatocyte differentiation protocol were able to more specifically store glycogen than undifferentiated HTASCs, displaying a purple color in the cytoplasm of the differentiated HTASCs. ELISA analyses showed that differentiated HTASCs could secrete albumin, which is one of the hepatocyte markers.
CONCLUSIONS
MSCs were islolated from human thigh adipose tissue differentiate to heapatocytes. The source of ADSCs is not only abundant abdominal adipose tissue, but also thigh adipose tissue for cell therapy in liver regeneration and tissue regeneration.
Keyword
MeSH Terms
-
Abdominal Fat
Adipose Tissue
Cell Differentiation
Collagen
Cytokines
Cytoplasm
Enzyme-Linked Immunosorbent Assay
Glycogen
Hepatocytes
Humans
Intercellular Signaling Peptides and Proteins
Liver Regeneration
Mesenchymal Stromal Cells
Periodic Acid
Polymerase Chain Reaction
Regeneration
Reverse Transcription
RNA, Messenger
Stem Cells
Thigh
Tissue Therapy
Collagen
Cytokines
Glycogen
Intercellular Signaling Peptides and Proteins
Periodic Acid
RNA, Messenger
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Adipose-derived stem cells: characterization and clinical application
- Recent Advances for Enhancing Drug Metabolizing Functions of Hepatocyte-like Cells Derived from Human Pluripotent Stem Cells
- Hepatogenic differentiation of human mesenchymal stem cells from peritoneal adipose tissue
- In vitro Differentiation of Human Mesenchymal Stem Cells into Hepatocytes
- In vitro Differentiation of Human Embryonic Stem Cells into Definitive Endodermal Cells
