Chonnam Med J.  2010 Dec;46(3):148-155. 10.4068/cmj.2010.46.3.148.

Antioxidant Activity of Rubus coreanus Fruit Extract: In Comparison to Green Tea Extract

Affiliations
  • 1Department of Biochemistry, Medical School and The Research Institute of Medical Sciences, Chonnam National University, Gwangju, Korea. syyang@jnu.ac.kr

Abstract

This study aimed to evaluate the antioxidant activity of Korean raspberry (Rubus coreanus Miq.) fruits, which are grown widely in the southwest area of Korea. Freeze-dried Rubus coreanus (RC) fruits and green tea (GT) were used to obtain water extracts (RCE and GTE, respectively). The total phenolic contents of the dried RC and GT were 126micromol CE (catechin equivalent)/g and 360micromol CE/g, respectively. The free radical scavenging activity of RCE and GTE was expressed as SC50 (50% scavenging capacity) and compared. The scavenging activity of RC toward DPPH, superoxide, and hydroxyl radicals was lower than that of GT. The inhibitory activity of RCE and GTE on AAPH-induced lipid peroxidation was also analyzed. In contrast with the free radical scavenging activities, the lipid peroxidation-inhibiting activity of RC was superior to that of GT. These results suggest that RCE contains effective scavengers of peroxyl radicals. In summary, RC contains less phenolics than does GT. Although RC has lower activity for scavenging DPPH, superoxide, and hydroxyl radicals than does GT, its activity to inhibit peroxyl radical-lipid peroxidation was higher. These results suggest that RC could be used as a valuable natural source of antioxidants.

Keyword

Rubus coreanus; Antioxidant activity; Free radicals

MeSH Terms

Antioxidants
Free Radicals
Fruit
Korea
Lipid Peroxidation
Phenol
Superoxides
Tea
Water
Antioxidants
Free Radicals
Phenol
Superoxides
Tea
Water

Figure

  • Fig. 1 Dpph Radical-scavenging Activity (A) And Sc50 (B) Of Gte And Rce. The Concentrations Of Gte And Rce Were Expressed As Catechin Equivalent (µm Ce). Each Value Is A Mean±s.D. (N=3). Sc50 Of Ascorbate, 30.16 Mm Ce; Sc50 Of Each Substance Was As Follows: Egcg, 6.52 Mm Ce; Gte, 15.93 Mm Ce; Rce, 17.68 Mm Ce. Egcg, (-)-epigallocatechin Gallate; Gte, Water Extract Of Green Tea; Rce, Te Water Extract Of Rubus Coreanus Fruits; Sc50, 50% Scavenging Capacity Of Total Radicals; Ce, Catechin Equivalent.

  • Fig. 2 Superoxide radical-scavenging activity (A) and Sc50 (B) of GTE and RCE. Superoxide radicals were generated from the reaction of 0.5 mM PMS and 0.15 mg Pi/mL PC liposomes. The concentrations of GTE and RCE were expressed as catechin equivalent (µM CE). Each value is a mean±S.D. (n=3). Sc50 of each substance was as follows: EGCG, 29.17 µM CE; GTE, 59.33 µM CE; RCE, 103.06 µM CE. Abbreviations: EGCG, (-)-Epigallocatechin gallate; GTE, water extract of green tea; RCE, water extract of Rubus coreanus fruits; Sc50, 50% scavenging capacity of total radicals; CE, catechin equivalent.

  • Fig. 3 Hydroxyl radical-scavenging activity (A) and Sc50 (B) of RCE and GTE. Hydroxyl radicals were generated by a mixed-function oxidation system containing 0.5 mM ascorbate and 50 µM Cu (II). Concentrations of GTE and RCE were expressed as catechin equivalent (µM CE). Each value is a mean±S.D. (n=3). Sc50 of each substance was as follows: EGCG, 29.92 µM CE; GTE, 39.34 µM CE; RCE, 35.39 µM CE. EGCG, (-)-Epigallocatechin gallate; GTE, water extract of green tea; RCE, water extract of Rubus coreanus fruits; Sc50, 50% scavenging capacity of total radicals; CE, catechin equivalent.

  • Fig. 4 Lipid peroxidation-inhibiting activity (A) and Sc50 (B) of RCE and GTE. Lipid peroxidation of PC liposomes was induced with 500 µM AAPH at 37℃ for 1 h. Concentrations of GTE and RCE were expressed as catechin equivalent (µM CE). Each value is a mean±S.D. (n=3). Sc50 of each substance was as follows: EGCG, 0.34µM CE; GTE, 0.70µM CE; RCE, 0.52µM CE. EGCG, (-)-Epigallocatechin gallate; GTE, water extract of green tea; RCE, water extract of Rubus coreanus fruits; Sc50, 50% scavenging capacity of total radicals; CE, catechin equivalent; MDA, malondialdehyde.


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