Korean J Blood Transfus.  2012 Aug;23(2):127-135.

Evaluation of IH-1000 for Automated ABO-Rh Typing and Irregular Antibody Screening

Affiliations
  • 1Department of Laboratory Medicine, College of Medicine, Chungnam National University, Daejeon, Korea. jmkim@cnuh.co.kr

Abstract

BACKGROUND
Despite modern advances in laboratory automated medicine, work-process in the blood bank is still handled manually. Several automated immunohematological instruments have been developed and are available in the market. The IH-1000 (Bio-Rad Laboratories, Hercules, CA, USA), a fully automated instrument for immunohematology, was recently introduced. In this study, we evaluated the performance of the IH-1000 for ABO/Rh typing and irregular antibody screening.
METHODS
In October 2011, a total of 373 blood samples for ABO/Rh typing and 303 cases for unexpected antibody screening were collected. The IH-1000 was compared to the manual tube and slide methods for ABO/Rh typing and to the microcolumn agglutination method (DiaMed-ID system) for antibody screening.
RESULTS
For ABO/Rh typing, concordance rate was 100%. For unexpected antibody screening, positive results for both column agglutination and IH-1000 were observed in 10 cases (four cases of anti-E and c, three of anti-E, one of anti-D, one of anti-M, and one of anti-Xg) and negative results for both were observed in 289 cases. The concordance rate between IH-1000 and column agglutination was 98.7%. Sensitivity and specificity were 90.9% and 99.3%, respectively.
CONCLUSION
The automated IH-1000 showed good correlation with the manual tube and slide methods and the microcolumn agglutination method for ABO-RhD typing and irregular antibody screening. The IH-1000 can be used for routine pre-transfusion testing in the blood bank.

Keyword

IH-1000; ABO typing; RhD typing; Antibody screening; Automation

MeSH Terms

Agglutination
Automation
Blood Banks
Isoantibodies
Mass Screening
Sensitivity and Specificity
Isoantibodies
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