Korean J Gastrointest Endosc.  2008 Jan;36(1):1-6.

Targeting Colon Cancer Cells with Fluorescent Magnetic Nanoparticles Conjugated to Anti-epidermal Growth Factor Receptor Antibodies

• Kim CW
• Cho YS
• Yoon TJ
• Kim HK
• Kim SS
• Chang KY
• Chae HS
• Choi MG
• Choi KY

Abstract

BACKGROUND/AIMS
The aim of this study was to evaluate targeting the epidermal growth factor receptors (EGFRs) of colon cancer cells with fluorescent magnetic nanoparticles (FMNP) conjugated to anti-EGFR antibodies. METHODS: The expression of EGFRs was evaluated in the HT-29 colon cancer cell lines and the control H-520 lung cancer cells by performing Western blot analysis. We synthesized silica-overcoated iron oxide nanoparticles that contained rhodamine B isothiocyanate (RITC) within a silica shell of a controllable thickness. This FMNP was conjugated to anti-EGFR mouse monoclonal antibody. The cells were treated with this probe conjugate for 4 hours and then the targeting was assessed via confocal microscopy. The fluorescence properties were evaluated for their binding to the expressed EGFRs with using a FACScan flow cytometer. RESULTS: EGFR was expressed in the HT-29 cells, as assessed by Western blot analysis. Red fluorescence was only detected in the membrane regions of the HT-29 cells on the confocal microscope imaging. On the FACS analysis, there was a significant shift of fluorescence intensity for the HT-29 cells. CONCLUSIONS: Our data show the feasibility of targeting colon cancer cells with FMNP conjugated with anti-EGFR antibodies in vitro.

Keyword

Colon cancer; Fluorescent magnetic nanoparticle (FMNP); EGFR

MeSH Terms

Animals
Antibodies
Blotting, Western
Cell Line
Colon
Colonic Neoplasms
Ferric Compounds
Fluorescence
HT29 Cells
Humans
Iron
Lung Neoplasms
Magnetics
Magnets
Membranes
Mice
Microscopy, Confocal
Nanoparticles
Receptor, Epidermal Growth Factor
Rhodamines
Silicon Dioxide
Antibodies
Ferric Compounds
Iron
Receptor, Epidermal Growth Factor
Rhodamines
Silicon Dioxide