J Korean Ophthalmol Soc.  1997 Dec;38(12):2129-2135.

The Effect of Transforming Growth Factor-beta and Mannose-6-Phosphate on the Proliferation of Subconjunctival Fibroblast of Rabbit

Affiliations
  • 1The Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea.
  • 2Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.

Abstract

The main cause of failure in glaucoma filtering surgery is obstruction of aqueous outflow due to subconjunctival fibrosis. Transforming growth factor-beta(TGF-beta) is known to be a growth factor for subconjunctival fibroblast. Recently, mannose-6-phosphate(M-6-P) is reported to be an inhibitor of TGF-beta activity. In this study, we evaluated the effects of TGF-betas and M-6-P on the proliferation of cultured subconjunctival fibroblast of white rabbit in vitro. Cell proliferation was determined by 3H-thymidine DNA incorporation method. TGF-beta1, 2, 3 all promoted proliferation of subconjunctival fibroblast in a concentration dependent fashion and the effect of TGF-beta1 was most prominent among 3 types. Low concentration (0.01mM) of M-6-P paradoxically increased cell proliferation, but with the concentration of 1.0mM, the inhibitory effects were varied in the range of 45% to 7%.

Keyword

Glaucoma filtration surgery; Mannose-6-phosphate; subconjunctival fibroblast; Transforming growth factor-beta
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