J Korean Orthop Assoc.  2011 Feb;46(1):18-27. 10.4055/jkoa.2011.46.1.18.

New Bone Formation Following Transplantation of Stem Cells and Nanoscale Hydroxyapatite Scaffold Materials into Rabbit Long Bone Defects

  • 1Department of Orthopaedic Surgery, Pusan National University Hospital, Busan, Korea. kimht@pusan.ac.kr
  • 2Department of Medicine, Graduate School, Pusan National University, Busan, Korea.
  • 3Medical Research Institute, Pusan National University Hospital, Busan, Korea.
  • 4Department of Pathology, Medical College, Kosin University, Busan, Korea.
  • 5Aerospace and Mechanical Engineering, Korea Aerospace University, Goyang, Korea.
  • 6Department of Advanced Materials Engineering, Chosun University, Gwangju, Korea.


We observed new bone formation following the transplantation of allogenic periosteum-derived stem cells and different sizes of hydroxyapatite (HA) scaffold materials into rabbit long-bone defects.
Thirty-two white rabbits were grouped according to the material transplanted into their tibial bone defects: group 1 (microscale HA only); group 2 (nanoscale HA only); group 3 (microscale HA plus stem cells); and group 4 (nanoscale HA plus stem cells). Viscosity was controlled by the relative amounts of HA and agar. After surgery, radiologic, microscopic, and biochemical observations were performed weekly for 8 weeks.
Nanoscale HA (groups 2 and 4) provided better bone formation than microscale HA (groups 1 and 3). The rabbits that had been transplanted with nanoscale HA plus stem cells (group 4) had more homogeneous bone formation during the natural repair process than the other groups.
Further study is required using nanoscale HA plus organic substance and stem cells, which are more similar to human bone structure, for better bone formation.


bone defect; stem cell; nanoscale hydroxyapatite
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