J Korean Soc Virol.  1999 Jun;29(2):137-144.

Characterization of Insect Cells Transformed with Autographa calfornica Nuclear Polyhedrosis Virus IE1 Gene

Affiliations
  • 1College of Natural Resources and Life Science, Dong-A University, Pusan 604-714, Korea. brjin@seunghak.donga.ac.kr
  • 2Department of Sericulture and Entomology, The National Institute of Agricultural Science and Technolgy, RDA, Suwon 441-744, Korea.

Abstract

Transformation efficiency, virus multiplication and foreign gene expression were characterized in the insect cells transformed with Autographa calfornica nuclear polyhedrosis virus (AcNPV) immediate early 1 gene (IE1). Transformation efficiency of insect cells by AcNPV IE1 gene vector horboring foreign gene was approximately 8-fold higher in the Sf9 cells transformed previously with AcNPV IE1 gene than in the normal Sf9 cells. Virus multiplication and foreign gene expression of recombinant baculovirus in the Sf9 cells transformed with AcNPV IE1 gene were similar to those of the normal Sf9 cells. These results suggest that transformed cells displaying foreign gene product by using AcNPV IE1 gene promoter will be useful for the diverse applications of insect cells.

Keyword

Insect cells; Baculovirus; Transformation; Expression

MeSH Terms

Baculoviridae
Gene Expression
Insects*
Nucleopolyhedrovirus*
Sf9 Cells
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