Korean J Anat.  2008 Dec;41(4):241-253.

The Properties of Glycoconjugates in the Rat Nasal Mucosa following Inhalation of Formaldehyde

Affiliations
  • 1Department of Anatomy, College of Medicine, Catholic University of Daegu, Daegu, Korea. ysmoon@cu.ac.kr

Abstract

The present study was carried out to investigate the glycoconjugate properties of the nasal mucosa in the rat after inhalation of formaldehyde. Sprague-Dawley male rats were inhalated 30 ppm formaldehyde for 3 times with 3 hours exposure. The olfactory and respiratory mucosa in the nasal mucosa were taken from the animals on 3, 6,9 days and 2, 3, 4, 5 weeks after inhalation of formaldehyde. The properties of glycoconjugate of the olfactory and respiratory mucosa were investigated using nine biotinylated lectins (PSA, UEA I, PHA-L, BSL I, PNA, MAL I, DBA, BSL II or sWGA). In experimental groups, the degenerative changes of the olfactory epithelium were observed until 3 weeks after inhalation of formaldehyde, but the respiratory epithelium was no change. In control group, the olfactory cells in the olfactory epithelium reacted with PSA, UEA I, PNA, DBA, BSL II, sWGA, and the supporting cells reacted with PSA, PHA-L, PNA, MAL I, DBA, BSL II, sWGA, and Bowman's glands reacted with all the lectins. In experimental groups, the olfactory cells reacted with UEA I, DBA, and the supporting cells reacted with PHA-L, MAL I, DBA, UEA I, and the positive reaction of Bowman's glands was increased. In control group, the goblet cells in the respiratory epithelium reacted with UEA I, MAL I, and the ciliated columnar cells reacted with PSA, UEA I, PHA-L, BSL I, DBA, BSL II, sWGA, and the septal nasal glands reacted with all the lectins except UEA I. In experimental groups, the goblet cells reacted with UEA I, MAL I and PNA. Conclusively, the olfactory mucosa was shown a lot of changes in the properties of glycoconjugates following inhalation of formaldehyde, but respiratory mucosa was shown feeble change. These results suggest that there were different sugar residues of glycoconjugate in the olfactory and respiratory mucosa following inhalation of formaldehyde, respectively.

Keyword

Glycoconjugates; Formaldehyde; Nasal mucosa; Olfactory epithelium; Respiratory epithelium; Lectin

MeSH Terms

Animals
Formaldehyde
Glycoconjugates
Goblet Cells
Humans
Inhalation
Lectins
Male
Nasal Mucosa
Olfactory Mucosa
Phytohemagglutinins
Rats
Respiratory Mucosa
Wheat Germ Agglutinins
Formaldehyde
Glycoconjugates
Lectins
Phytohemagglutinins
Wheat Germ Agglutinins
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