Korean J Blood Transfus.  2004 Jun;15(1):15-22.

Comparison of Coagulation Factor Activities of Cryoprecipitate-depleted plasma(CDP) Prepared by Two Different Methods

• Lee MK
• Kwon SY
• Oh YC
• Chi HS

Abstract

BACKGROUND: Cryoprecipitate depleted plasma(CDP) that is selectively used for therapeutic plasma exchange for the thrombotic thrombocytopenic purpura(TTP). We evaluated coagulation factor activities of CDP prepared by two different methods to use CDP as a blood component and induce a new guideline.
METHODS
We studied 32 units of CDP were made from FFP collected from 16 donors. To prepare CDP, units of FFP were thawed in a 4degrees C water bath for 1 to 2 hours(rapid thawing) or thawed in a 1 to 6degrees C refrigerator for 14 to 16 hours(overnight thawing). Then CDP were refrozen within 1 hours, stored at -45degrees C and re-thawed in three weeks later. We measured prothrombin time(PT), activated partial prothrombin time(aPTT), fibrinogen, coagulation factor V, VIII, von willebrend factor(vWF) and vWF multimer of CDP at the time of preparation, after re-freezing and re-thawing. And we compared them with the results of platelet poor plasma(PPP).
RESULTS
The mean volume of CDP was 129+/-12mL. We found all measured factors of CDP except factor V were significantly lower or longer than those of PPP. In the comparision of thawing method, rapid thawing CDP showed longer PT, higher activities of fibrinogen and factor VIII, lower activities of vWF than those of overnight thawing with no significant change of factor V. No significant changes were notified in all factors between the results of CDP within 1 hour of preparation and those of after re-freezing and re-thawing.
CONCLUSIONS
CDP prepared by rapid thawing is more recommendable for therapeutic plasma exchange for TTP. CDP prepared by re-freezing and re-thawing can be used as a CDP just prepared from FFP.

Keyword

Cryoprecipitate depleted plasma(CDP); Thrombotic thrombocytopenic purpura(TTP); Coagulation factor

MeSH Terms

Baths
Blood Coagulation Factors*
Blood Platelets
Cytidine Diphosphate
Factor V
Factor VIII
Fibrinogen
Humans
Plasma Exchange
Prothrombin
Tissue Donors
Blood Coagulation Factors
Cytidine Diphosphate
Factor V
Factor VIII
Fibrinogen
Prothrombin