Korean J Dermatol.  1986 Feb;24(1):1-7.

Separation of Human Epidermal Cells by Using Percoll

Abstract

The epidermis, which is composed mainly of keratinocyt;es, undergoes continual proliferation and differentiation, To study keratinocyte differentiation, it is necessary to separate the keratinocytes at their sucessive stages of maturation. We separate the keratinocytes by layers by using Percoll and obtain the relatively pure fractions of basal cells, spinous cells, and granular cells, 76. 8%, 67. 2%, 30%. respectively.

Keyword

Percoll; Separation of human epidermal cells; Density gradient1

MeSH Terms

Epidermis
Humans*
Keratinocytes
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