Korean J Nephrol.  2005 Jan;24(1):19-25.

Non-Surgical Gene Transfer to Rabbit Renal Glomeruli via Percutaneous Arterial Catheterization

• Park BH
• Hwang TH
• Kim SE
• Kim KH
• Kong GS
• Jun HS
• Jeong JS
• Rha SH

Abstract

BACKGROUND
Transfer of foreign genes to the renal glomerular cells is an important step for the gene therapy of renal diseases in which the primary pathology is confined to the glomeruli. We developed a non-surgical method of gene transfer to rabbit renal glomeruli using percutaneous arterial catheterization without any laparatomy procedure. METHODS: The recombinant adenovirus type 5, containing a nuclear-targeted beta-galactosidase gene and driven by a cytomegalovirus promoter, was slowly infused into the unilateral renal artery via percutaneous arterial catheterization. The animals were sacrificed 3 days after virus infusion and lacZ staining was done on the fresh harvested tissue. RESULTS: Only the animals those received 6x10(12) particles/rabbit for 120 minutes show lacZ expression in 90.6+/-5% (n=3) of glomeruli. Mostly, it was the endothelial cells and mesangial cells those were positive for the stain. CONCLUSION: This non-surgical method for gene transduction of the renal glomeruli can be applied to human trials of glomerulus-directed gene therapy.

Keyword

Glomerulus; Gene transfer; Adenovirus

MeSH Terms

Adenoviridae
Animals
beta-Galactosidase
Catheterization*
Catheters*
Cytomegalovirus
Endothelial Cells
Genetic Therapy
Humans
Mesangial Cells
Pathology
Renal Artery
beta-Galactosidase