Korean J Pain.  2014 Apr;27(2):118-124. 10.3344/kjp.2014.27.2.118.

Acute Systemic Infusion of Bupropion Decrease Formalin Induced Pain Behavior in Rat

Affiliations
  • 1Danesh Pey Hadi Co., Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
  • 2Neurophysiology Resaerch Center, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran. info@fgpakdel.com
  • 3Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
  • 4Department of Histology & Embryology, School of Medicine, Dokuz EyluL University (DEU), Izmir, Turkey.

Abstract

BACKGROUND
The chronic pain can disturb physical, psychological, and social performances. Analgesic agents are widely used but some antidepressants (ADs) showed analgesia also. Bupropion is using for smoke cessation but it can change morphine withdrawal signs such as pain. This study tested the acute systemic effect of bupropion on formalin induced pain behavior in rats.
METHODS
Wistar male healthy rats were divided into 7 groups (control, sham, and 5 treated groups with 10, 30, 90, 120, and 200 mg/kg of bupropion, i.p.). The bupropion injected 3 hours prior to formalin induced pain behavior. Formalin (50 microl, 2.5%) was injected subcutaneously in dorsal region of right hindpaw in all animals. Nociceptive signs were observed continuously on-line and off-line each minute. Common pain scoring was used for pain assessment.
RESULTS
The analysis of data by one-way ANOVA showed that bupropion can reduce pain scores in the second phase but not in first phase. Bupropion decreased the licking/biting duration significantly in first and second phase of formalin test.
CONCLUSIONS
The results showed that bupropion has analgesic effects at systemic application. The change of second phase of the pain behavior was significant and it revealed that central mechanisms involve in bupropion analgesia.

Keyword

analgesia; antidepressant; bupropion; formalin test; pain; rat

MeSH Terms

Analgesia
Analgesics
Animals
Antidepressive Agents
Bupropion*
Chronic Pain
Formaldehyde*
Humans
Male
Morphine
Pain Measurement
Rats*
Smoke
Analgesics
Antidepressive Agents
Bupropion
Formaldehyde
Morphine
Smoke

Figure

  • Fig. 1 (A) The formalin induced pain scores in rats. The all doses of bupropion injected 3 hours before formalin test. Each point represents the mean ± SEM (n = 6) number of pain scores during 5 min observation period. There was no significant difference between control, sham, and 10 mg/kg of bupropion groups. There was significant difference between, sham and doses of 30, 90, 120, and 200 mg/kg of bupropion groups. (B) The cumulative formalin induced pain score in rats. The all doses of bupropion injected 3 hours before formalin test. Cumulative formalin induced pain scores of first phase between all groups had no significant difference but the second phase had significant difference in doses of 30, 90, 120, and 200 mg/kg of bupropion groups in comparison with sham group (one-way repeated measured ANOVA, Tukey's post hoc test, ***P < 0.001, **P < 0.01, and *P < 0.05).

  • Fig. 2 (A) The formalin induced licking/biting duration in rats. The all doses of bupropion injected 3 hours before formalin test. Each point represents the mean ± SEM (n = 6) number of licking/biting duration during 5 min observation period. There was no significant difference between control, sham, 10, and 30 mg/kg of bupropion groups. There was significant difference between, sham and doses of 90, 120, and 200 mg/kg of bupropion groups. (B) The cumulative formalin induced licking/biting duration in rats. The all doses of bupropion injected 3 hours before formalin test. Cumulative formalin induced licking/biting of phase 01 between groups had no significant difference in groups control, sham, 10, and 30 mg/kg of bupropion. In the phase 02 there is a significant difference between sham and all bupropion treated groups (one-way repeated measured ANOVA, Tukey's post hoc test, ***P < 0.001, **P < 0.01, and *P < 0.05).


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