Korean J Physiol Pharmacol.  2010 Oct;14(5):325-329. 10.4196/kjpp.2010.14.5.325.

Epigallocatechin-3-gallate Regulates NADPH Oxidase Expression in Human Umbilical Vein Endothelial Cells

• Ahn HY
• Kim CH
• Ha TS

• KMID: 2071701
• DOI: http://doi.org/10.4196/kjpp.2010.14.5.325

Abstract

Vascular NADPH oxidase plays a pivotal role in producing superoxide in endothelial cells and thus acts in the initiation and development of inflammatory cardiovascular diseases such as atherosclerosis. Epigallocatechin-3-gallate (EGCG), the major catechin derived from green tea, has multiple beneficial effects for treating cardiovascular disease but the effect of EGCG on the expression of vascular NADPH oxidase remains unknown. In this study, we investigated the mechanism(s) by which EGCG might inhibit the expression of subunits of NADPH oxidase, namely p47(phox), p67(phox) and p22(phox), induced by angiotensin II (Ang II) in human umbilical vein endothelial cells. Ang II increased the expression levels of p47(phox), p67(phox), and p22(phox), but EGCG counteracted this effect on p47(phox). Moreover, EGCG did not affect the production of reactive oxygen species induced by Ang II. These data suggest a novel mechanism whereby EGCG might provide direct vascular benefits for treating inflammatory cardiovascular diseases.

Keyword

EGCG; NADPH oxidase; Angiotensin II; ROS; HUVEC

MeSH Terms

Angiotensin II
Atherosclerosis
Cardiovascular Diseases
Catechin
Endothelial Cells
Human Umbilical Vein Endothelial Cells
Humans
NADP
NADPH Oxidase
Reactive Oxygen Species
Superoxides
Tea
Angiotensin II
Catechin
NADP
NADPH Oxidase
Reactive Oxygen Species
Superoxides
Tea

Figure

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  Fig. 1. Effect of Ang II treatment (100 nM, 0∼24 h) on the expression levels of NADPH oxidase subunits p47phox, p67phox and p22phox in HUVECs. Summary data are shown as the mean±SEM. ∗p<0.05 by Turkey's multiple comparison test.

• 

  Fig. 2. Effect of EGCG on p47phox expression in HUVECs treated with Ang II (100 nM) for 24 h, 30 min after treatment with EGCG (10∼50 μM).

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  Fig. 3. Effect of EGCG (30–50 μM) on ROS induced by Ang II (100 nM) for 24 h in HUVECs. EGCG was pretreated 30 min before Ang II stimulation. Bars represent the mean±SEM. ∗p<0.05 by Turkey's multiple comparison test.

Reference

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