Korean J Physiol Pharmacol.
2001 Aug;5(4):333-342.
The protective effect of melatonin administration against adriamycin-induced cardiotoxicity in rats
- Affiliations
-
- 1Department of Physiology and Biophysics, College of Medicine, Inje University, Busan, South Korea. phykimey@ijnc.inje.ac.kr
Abstract
- Adriamycin is a commonly used chemotherapeutic agent for cancer, including acute leukemia, lymphoma, and a number of solid human tumors. However, recent studies have recognized severe cardiotoxicity after an acute dose, which are likely the result of generation of free radicals and lipid peroxidation. Therefore, the clinical uses of adriamycin have been limited. Melatonin, the pineal gland hormone known for its ability to modulate circardian rhythm, has recently been studied in its several functions, including cancer growth inhibition, stimulating the immune system, and acting as an antioxidant and radical scavenging effects. In the present study, we evaluated the effect of melatonin administration on adriamycin-induced cardiotoxicity in rat. Heart slices were prepared using a Stadie-Riggs microtome for the measurement of malondialdehyde (MDA) content used as an index of lipid peroxidation and lactate dehydrogenase (LDH) release as an indicator of lethal cell injury. Serious adriamycin-induced lethality was observed in rat by a single intraperitoneal injection in a dose-dependent manner. A single injection of adriamycin (25 mg/kg, i.p.) induced a lethality rate of 86%, with melatonin (10 mg/kg s.c. for 6 days) treatment reducing the adriamycin-induced lethality rate to 20%. The severe body weight loss caused by adriamycin was also significantly attenuated by melatonin treatment. Treatment of melatonin marked reduced adriamycin-induced the levels of MDA formation and LDH release. A cell damage indicated by the loss of myofibrils, swelling of the mitochondria as well as cytoplasmic vacuolization was seen in adriamycin-treated group. Melatonin attenuated the adriamycin-induced structural alterations. These data provide evidence that melatonin prevents adriamycin-induced cardiotoxicity and might serve as a combination with adriamycin to limit free radical-mediated cardiotoxicity.
MeSH Terms
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- The Role of Apoptosis in Adriamycin Induced Cardiotoxicity and Preventive Effect of L-carnitine in Rat
- Protective Effect of Melatonin on Neuropathy in Streptozotocin-Induced Diabetic Rats
- A Study of Glomerulonephropathy Induced by Adriamycin in Rats
- Effect of Melatonin on the Diabetes Mellitus Induced by Streptozotocin in Rats
- Effect of Sucralfate of the Prophylaxia of Adriamycin Induced Mucositis in the Rat
