Korean J Physiol Pharmacol.  2001 Aug;5(4):299-305.

Regulation of phosphorylated cAMP response element-binding protein, Fos-related antigen and FosB expression by dopamine agonists in rat striatum

• Choe ES
• Kim JY

Abstract

Activation of D1-like dopamine receptors by psychostimulants, such as amphetamine, upregulates the expression of immediate early gene and opioid peptide gene in the striatum. The genomic changes are regulated by phosphorylated transcription factors via complicated intracellular events. To evaluate temporal expression of the transcription factors by dopaminergic stimulation, the D1-like dopamine agonist, amphetamine or SKF82958, was systematically delivered. As intracellular markers in response to the agonist, phosphorylated cAMP response element-binding protein (pCREB), Fos-related antigens (FRA) and FosB immunoreactivity (IR) was compared at 20 and 120 min time points in the selected areas of the striatum. Semi-quantitative immunocytochemistry showed that amphetamine (5 mg/kg, i.p.) significantly increased pCREB-IR at 20 min, sustained up to 60 min and decreased at 120 min after the infusion. Like amphetamine, the full D1 agonist, SKF82958 (0.5 mg/kg, s.c.), also increased pCREB-IR at 20 min, but not at 120 min after the infusion in the dorsal striatum (caudoputaman, CPu) and shell of ventral striatum (nucleus accumbens, NAc). In contrast, FRA- and FosB-IR induced by SKF82958 was significantly increased at 120 min, but not at 20 min after the administration. These data indicate that SKF82958 mimics induction of CREB phosphorylation by amphetamine and differentially regulates temporal induction of pCREB, and FRA and FosB expression in the striatum.

MeSH Terms

Amphetamine
Animals
Basal Ganglia
Cyclic AMP Response Element-Binding Protein*
Dopamine Agonists*
Dopamine*
Immunohistochemistry
Opioid Peptides
Phosphorylation
Proto-Oncogene Proteins c-fos
Rats*
Receptors, Dopamine
Transcription Factors
Amphetamine
Cyclic AMP Response Element-Binding Protein
Dopamine
Dopamine Agonists
Opioid Peptides
Proto-Oncogene Proteins c-fos
Receptors, Dopamine
Transcription Factors