Korean J Physiol Pharmacol.  1998 Oct;2(5):549-553.

Dual action of d-tubocurarine on large-conductance Ca2+-activated K+ channels from rat brain reconstituted into planar lipid bilayer

Affiliations
  • 1Department of Physiology, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
  • 2Department of Physiology and Biophysics, Inha University College of Medicine, Inchon 402-751, Korea.

Abstract

Using the planar lipid bilayer method, we investigated the effect of d-tubocurarine (dTC) on the extracellular side of large-conductance Ca2+-activated K+ channel from rat brain. When the initial open probability (Po) of the channel was relatively high, dTC decreased channel activity in a concentration dependent manner. In contrast, when the initial Po was lower, sub-micro molar dTC increased channel activity by destabilizing the closed states of the channel. Further addition of dTC up to micro molar range decreased channel activity. This dual effect of dTC implicates that there exist at least two different binding sites for dTC.

Keyword

Ca2+-activated k+Channel; d-Tubocurarine; Planar lipid bilayer
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