J Vet Sci.  2014 Dec;15(4):495-502. 10.4142/jvs.2014.15.4.495.

M gene analysis of canine coronavirus strains detected in Korea

• Jeoung SY
• Ann SY
• Kim HT
• Kim D

• KMID: 2070235
• DOI: http://doi.org/10.4142/jvs.2014.15.4.495

Abstract

The purpose of this study was to investigate the genetic features of canine coronavirus (CCV) strains detected in Korea. M gene sequences obtained for isolates from 22 dogs with enteritis over a 5-year period were evaluated. Sequence comparison revealed that the 22 Korean CCV strains had an 87.2 to 100% nucleotide homology. Comparing to the typical reference CCV strains (type II), the nucleotide sequence of Korean strains had homology ranged from 86.3% to 98.3% (89.1% to 99.2% for the amino acid sequence) and 87.7% to 97.8% (92.4% to 100% for the amino acid sequence) when compared to FCoV-like CCV strains (type I). Three amino acid variations in the M gene were characteristic for the Korean CCV strains. Phylogenetic analysis demonstrated that the 22 Korean CCV strains belonged to four typical CCV clusters (i.e., a unique Korean CCV cluster, a type II and transmissible gastroenteritis virus cluster, an intermediate cluster between type I and II, and a type I cluster). This study was the first to identify genetic differences of the M gene from Korean CCV strains and provided a platform for molecular identification of different Korean CCV strains.

Keyword

canine coronavirus; M gene; phylogenetic analysis

MeSH Terms

Amino Acid Sequence
Animals
Coronavirus Infections/epidemiology/*veterinary/virology
Coronavirus, Canine/*isolation & purification
Dog Diseases/*epidemiology/virology
Dogs
Female
Male
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction/veterinary
Republic of Korea/epidemiology
Reverse Transcriptase Polymerase Chain Reaction/veterinary
Viral Matrix Proteins/*genetics/metabolism
Viral Matrix Proteins

Figure

• 

  Fig. 1 Phylogenetic tree of the CCVs based on a comparison of partial M gene amino acid sequences obtained for 22 Korean strains and 10 reference coronaviruses. CI, cluster I; CII, cluster II; CIII, cluster III; and CIV, cluster IV.

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