Korean J Blood Transfus.
1998 Dec;9(2):273-282.
Analysis of some important variables for quality improvement of platelet concentrate
- Affiliations
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- 1Department of Clinical Pathology, Hyundai Medical Laboratory.
- 2Asan Medical Center, Hyundai Medical Laboratory.
- 3Department of Clinical Pathology, SungkyunKwan University College of Medicine, Samsung Medical Center, Seoul, Korea.
- 4Department of Clinical Pathology, Yonsei University College of Medicine, Seoul, Korea.
- 5Department of Clinical Pathology, Seoul National University College of Medicine, Seoul, Korea.
Abstract
- BACKGROUND
Recent studies suggested that platelets can be activated during the preparation of platelet concentrates (PCs). We designed this study to assess the amount of platelet activation induced by some varialbles during the preparation and storage for the purpose of quality improvement of PCs.
METHODS
Using 66 fresh whole blood, we analysed the effects of the variables including the delayed time before preparation of PCs, centrifugal force, pH, 1-hour incubation at room temperature before platelet resuspension and platelet agitation stop. After preparation of PCs under various conditions, the percentage of p-selectin-positive platelets was determined by flowcytometry using monoclonal antibodies CD62.
RESULTS
The effects of delayed time within 8 hours before preparation and omission of 1-hour incubation on activaton of platelets were minimal. However, the effects of centrifugation speed and time were very significant. After centrifugation of platelet-rich plasma at 5,000 g for 5 or 7 minutes, percentage of p-selectin-positive platelets was increased up to 40.6% or 72.6% on day 1, and 59.7% or 87.7% on day 5, respectively. A good correlation was observed between pH and percentage of p-selectin expressed platelets (r=-0.8584, P=0.0001 on day 1; r=-0.7199, P=0.0037, respectively). We also observed that platelet agitation stop for 6 hours could activate platelets in PCs significantly.
CONCLUSION
High centrifugal force and platelet agitation stop were important variables which could activate platelets significantly during preparation and storage of PCs.
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