Korean J Urol.  1994 Apr;35(4):347-356.

Immunohistochemical Study of Ras and Epidermal Growth Factor Receptor Expression in BBN Induced Bladder Tumor of Rat

• Lee HS
• Kang SJ

Abstract

There is increasing evidence that genes encoding for signal transduction pathway, including ras and epidermal growth factor receptor( EGFR), are involved not only in normal cellular growth and differentiation but also in development and progression of malignant tumors. N-butyl-N- (4-hydroxybutyl) nitrosamine (BBN)-induced rat bladder tumor is an ideal model to study the time related gene expression in stepwise bladder epithelial carcinogenesis. We herein investigated the roles of ras and EGFR by immunohistochemical study in bladder tissues of Sprague-Dawley rats after administration of BBN. Sequential epithelial changes consisting of normal, simple hyperplasia, nodular or papillary hyperplasia, papilloma and superficial transitional cell carcinoma(TCC) were observed in rat bladders in parallel with the duration of BBN administration. The expressions of both ras and EGFR were weak in bladder epithelia of non-BBN-administered rats, whereas, immunoreactivity and number of reactive cell layer were increased in bladder epithelia of BBN-administered rats in parallel with the duration of BBN administration and progression of bladder epithelial changes. These results further support the hypothesis that ras and EGFR may have important roles in development of bladder TCC.

Keyword

BBN induced bladder tumor; Ras; EGFR; Immunohistochemistry

MeSH Terms

Animals
Carcinogenesis
Epidermal Growth Factor*
Gene Expression
Hyperplasia
Immunohistochemistry
Papilloma
Rats*
Rats, Sprague-Dawley
Receptor, Epidermal Growth Factor*
Signal Transduction
Urinary Bladder Neoplasms*
Urinary Bladder*
Epidermal Growth Factor
Receptor, Epidermal Growth Factor