Korean J Asthma Allergy Clin Immunol.  2011 Sep;31(3):199-206.

Anti-inflammatory and Anti-pruritic Effects of Portulaca oleracea L. Extract Using In Vitro and In Vivo Inflammation Model: LPS-treated Raw264.7 Cells, Keratinocytes, NC/Nga Mice and Hairless SKH-1 Mice

Abstract

BACKGROUND
Portulaca oleracea L. is known to have detoxification and antibacterial effects. Inflammatory responses in LPS-treated Raw264.7 cells include the secretions of NO, PGE2 and numerous cytokines. Atopic dermatitis is a chronic inflammatory skin disease. This study was performed to identify the antiinflammatory or anti-pruritic effects of Portulaca oleracea L. extract in LPS-treated Raw264.7 cells and keratinocytes and the skin of NC/Nga mice with atopic dermatitis, as well as hairless mice with pruritus.
METHODS
After the treatment of different concentrations for Portulaca oleracea L. extract in LPS-treated Raw264.7 cells and keratinocytes, the levels of NO, PGE2 and proinflammatory cytokines were measured in the media. Skin tissues of all Nc/Nga mice were subjected to H&E staining and toluidine blue staining. An antipruritic effect was defined as the number of scratching behaviors of the hairless SKH-1 mice treated with compound 48/80.
RESULTS
Results: LPS- and IFN-gamma-treated Raw264.7 cells decreased NO and PGE2 productions compared to non-treated Raw264.7 cells. H&E or toluidine blue staining revealed that the thicknesses of the epidermis and dermis were reduced in NC/Nga mice with atopic dermatitis treated with Portulaca oleracea L. extract. However, Itching behaviors in hairless mice treated with Portulaca oleracea L. extract were not decreased compared to non-treated hairless mice.
CONCLUSION
The results of this study suggest that Portulaca oleracea L. extract could be a new therapeutic agent for atopic dermatitis.


MeSH Terms

Animals
Cytokines
Dermatitis, Atopic
Dermis
Dinoprostone
Epidermis
Inflammation
Keratinocytes
Mice
Mice, Hairless
Portulaca
Pruritus
Skin
Skin Diseases
Tolonium Chloride
Cytokines
Dinoprostone
Tolonium Chloride
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