J Korean Assoc Oral Maxillofac Surg.  2005 Feb;31(1):7-12.

Anticancer effect of CKD-602(belotecan, camtobell(R)) on the oral cancer cell lines

• Yun PY
• Ok YJ
• Myoung H
• Lee JH
• Kim MJ

Abstract

PURPOSE: CKD-602, a newly developed water-soluble campthotecin analogue, is a anticancer agent which act as a DNA topoisomerase I inhibitor. CKD-602 is known as more potent and tolerable agent. The main purposes of this study were to measure the cytotoxic effect of CKD-602 on the oral cancer cell lines and to evaluate the apoptotic aspect of dead cells.
MATERIALS AND METHODS
To determine the cytotoxic effect of CKD-602 on the oral cancer cell lines in comparison with various cell lines, such as lung cancer and colon cancer cell lines, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was performed. And apoptosis was analyzed using fluorescence-activated cell sorting(FACS) system.
RESULTS
CKD-602 decreased the viability of malignant cells in a dose dependent manner and in a time dependent manner. CKD-602 showed excellent cytotoxicity to the oral cancer cell lines. Also, apoptotic portion was increased in a dose dependent manner.
CONCLUSION
These findings indicated that CKD-602 induced apoptotic cell death in the various cell lines including oral cancer cell lines. From the results, it was suggested that CKD-602 would be a potential therapeutic agent for the oral cancer. More successive researches on the anticancer effect of CKD-602 should be performed.

Keyword

CKD-602; Camptothecin; Topoisomerase-I inhibitor; Oral cancer; Apoptosis

MeSH Terms

Apoptosis
Camptothecin
Cell Death
Cell Line*
Colonic Neoplasms
DNA Topoisomerases, Type I
Lung Neoplasms
Mouth Neoplasms*
Camptothecin
DNA Topoisomerases, Type I