J Bacteriol Virol.  2004 Mar;34(1):57-66.

Immunodeficiency Virus Type 1 Infection of H9 Cells

Affiliations
  • 1Center for AIDS Research, Department of Virology, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Korea. jooshil@nih.go.kr
  • 2Genomic Tree Company, Daeduk Biocommunity, Daejun, Korea.

Abstract

Human immunodeficiency virus type 1 (HIV-1) virus causes severe defect in the immune system and affects the host cell gene expression profoundly. The gene expression pattern will be characterized by changes in cellular mRNA levels that are dependent on both the stage of infection and the biological state of the infected cells. The expression levels of 7,404 cellular RNA transcripts were assessed in H9 cells at different time points after HIV-1 IIIB infection. In total 7 time-points, 959/7,404 (13%) genes were a 2-fold or greater expressed. 387 of 959 genes (40.4%) were up-regulated, and other 572 genes (59.6%) were down-regulated. Three hundred seventeen genes were up-regulated a 2-fold or greater at 72 hr postinfection and 2 to 139 genes were up-regulated at the other time-points. In contrast, 126 to 349 genes were down-regulated a 2-fold or greater in all time-points, excepting 6 hr postinfection. Twenty-three genes were up-regulated a 2-fold or greater over at least four of seven time-points, which were mostly ribosomal proteins and MHCs. Especially, MHCs including HLA-DRA were steadily up-regulated from 24 hr postinfection. Thirty genes were down-regulated a 2-fold or greater in all the time-points, which were mainly related with synthesis and metabolism. These results show that host cell gene expression was altered by HIV-1 infection according to time-points and will provide a framework for studies on interactions between host and HIV-1 infection.

Keyword

HIV-1; Host cell; cDNA microarrays; Gene expression

MeSH Terms

Gene Expression
HIV-1
HLA-DR alpha-Chains
Immune System
Metabolism
Oligonucleotide Array Sequence Analysis
Ribosomal Proteins
RNA
RNA, Messenger
HLA-DR alpha-Chains
RNA
RNA, Messenger
Ribosomal Proteins
Full Text Links
  • JBV
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error