Mycobiology.  2009 Mar;37(1):31-36. 10.4489/MYCO.2009.37.1.031.

Antimicrobial and Cytotoxic Activity of Di-(2-ethylhexyl) Phthalate and Anhydrosophoradiol-3-acetate Isolated from Calotropis gigantea (Linn.) Flower

Affiliations
  • 1Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205, Bangladesh. rezaplazad@yahoo.com

Abstract

A phytochemical study on the flowe r of Calotropis gigantea (Linn.) using silica gel column chromatography and preparative thin layer chromatography, led to the first time isolation of Di-(2-ethylhexyl) phthalate (compound 1) and anhydrosophoradiol-3-acetate (compound 2). The structures of these compounds were confirmed by spectroscopic analyses (IR, HRTOFMS and NMR). The antibacterial and antifungal activities of ethyl acetate extract, compound 1 and compound 2 were measured using the disc diffusion method. Ethyl acetate extract and compound 1 presented better results than compound 2. The minimum inhibitory concentrations (MICs) of the extract and compounds were found to be in the range of 16~128 microg/ml. The cytotoxicity (LC50) against brine shrimp nauplii (Artemia salina) were also evaluated and found to be 14.61 microg/ml for ethyl acetate, 9.19 microg/ml for compound 1 and 15.55 microg/ml for compound 2.

Keyword

Anhydrosophoradiol-3-acetate; Antimicrobial; Calotropis gigantea and Di-(2-ethylhexyl) phthalate

MeSH Terms

Acetates
Artemia
Calotropis
Chromatography
Chromatography, Thin Layer
Diffusion
Flowers
Microbial Sensitivity Tests
Phthalic Acids
Salts
Silica Gel
Acetates
Phthalic Acids
Salts
Silica Gel
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