Tuberc Respir Dis.  2014 Nov;77(5):203-208. 10.4046/trd.2014.77.5.203.

Effects of Lobetyolin, Lobetyol and Methyl linoleate on Secretion, Production and Gene Expression of MUC5AC Mucin from Airway Epithelial Cells

• Yoon YP
• Ryu J
• Park SH
• Lee HJ
• Lee S
• Lee SK
• Kim JO
• Hong JH
• Seok JH
• Lee CJ

• KMID: 2050712
• DOI: http://doi.org/10.4046/trd.2014.77.5.203

Abstract

BACKGROUND
In this study, we investigated whether lobetyolin, lobetyol, and methyl linoleate derived from Codonopsis pilosula affect MUC5AC mucin secretion, production, and gene expression from airway epithelial cells.
METHODS
Confluent NCI-H292 cells were pretreated with lobetyolin, lobetyol, or methyl linoleate for 30 minutes and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. The MUC5AC mucin gene expression, and mucin protein production and secretion were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.
RESULTS
Lobetyolin, lobetyol, and methyl linoleate inhibited the gene expression of MUC5AC mucin induced by PMA; lobetyolin did not affect PMA-induced MUC5AC mucin production. However, lobetyol and methyl linoleate inhibited the production of MUC5AC mucin; lobetyolin and lobetyol did not significantly affect PMA-induced MUC5AC mucin secretion from NCI-H292 cells. However, methyl linoleate decreased the MUC5AC mucin secretion.
CONCLUSION
These results suggest that among the three compounds, methyl linoleate can regulate gene expression, production, and secretion of MUC5AC mucin by directly acting on the airway epithelial cells.

Keyword

Mucins; MUC5AC Protein, Human; Methyl Linoleate

MeSH Terms

Codonopsis
Enzyme-Linked Immunosorbent Assay
Epithelial Cells*
Gene Expression*
Linoleic Acid*
Mucins*
Polymerase Chain Reaction
Reverse Transcription
Linoleic Acid
Mucins

Figure

• 

  Figure 1 Effect of lobetyolin (A), lobetyol (B), or methyl linoleate (C) on phorbol 12-myristate 13-acetate (PMA)-induced MUC5AC gene expression from NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of lobetyolin, lobetyol, or methyl linoleate for 30 minutes and then stimulated with PMA (10 ng/mL) for 24 hours. MUC5AC gene expression was measured by reverse transcription polymerase chain reaction. Three independent experiments were performed and the representative data are shown. Met Lin: methyl linoleate.

• 

  Figure 2 Effect of lobetyolin (A), lobetyol (B), or methyl linoleate (C) on phorbol 12-myristate 13-acetate (PMA)-induced MUC5AC mucin production from NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of lobetyolin, lobetyol, or methyl linoleate for 30 minutes and then stimulated with PMA (10 ng/mL) for 24 hours. Cell lysates were collected for measuring MUC5AC mucin production by enzyme-linked immunosorbent assay. Three independent experiments were performed and the representative data are shown. Each bar represents a mean±SEM. of three culture wells in comparison with that of the control set at 100%. *Significantly different from control (p<0.05). †Significantly different from PMA alone (p<0.05). Concentration unit is µM.

• 

  Figure 3 Effect of lobetyolin (A), lobetyol (B), or methyl linoleate (C) on phorbol 12-myristate 13-acetate (PMA)-induced MUC5AC mucin secretion from NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of lobetyolin, lobetyol, or methyl linoleate for 30 minutes and then stimulated with PMA (10 ng/mL) for 24 hours. Spent media were collected for measuring MUC5AC mucin secretion by enzyme-linked immunosorbent assay. Three independent experiments were performed and the representative data are shown. Each bar represents a mean±SEM of three culture wells in comparison with that of the control set at 100%. *Significantly different from control (p<0.05). †Significantly different from PMA alone (p<0.05). Concentration unit is µM.

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