Biomol Ther.  2014 Jan;22(1):41-46.

Antiviral Activity of Hederasaponin B from Hedera helix against Enterovirus 71 Subgenotypes C3 and C4a

• Song J
• Yeo SG
• Hong EH
• Lee BR
• Kim JW
• Kim J
• Jeong H
• Kwon Y
• Kim H
• Lee S
• Park JH
• Ko HJ

Abstract

Enterovirus 71 (EV71) is the predominant cause of hand, foot and mouth disease (HFMD). The antiviral activity of hederasaponin B from Hedera helix against EV71 subgenotypes C3 and C4a was evaluated in vero cells. In the current study, the antiviral activity of hederasaponin B against EV71 C3 and C4a was determined by cytopathic effect (CPE) reduction method and western blot assay. Our results demonstrated that hederasaponin B and 30% ethanol extract of Hedera helix containing hederasaponin B showed significant antiviral activity against EV71 subgenotypes C3 and C4a by reducing the formation of a visible CPE. Hederasaponin B also inhibited the viral VP2 protein expression, suggesting the inhibition of viral capsid protein synthesis.These results suggest that hederasaponin B and Hedera helix extract containing hederasaponin B can be novel drug candidates with broad-spectrum antiviral activity against various subgenotypes of EV71.

Keyword

Enterovirus 71; Antiviral activity; Hederasaponin B; Hedera helix; Hand foot and mouth disease

MeSH Terms

Blotting, Western
Capsid Proteins
Enterovirus*
Ethanol
Hand, Foot and Mouth Disease
Hedera*
Vero Cells
Capsid Proteins
Ethanol