Anat Cell Biol.  2013 Mar;46(1):68-78. 10.5115/acb.2013.46.1.68.

Expression of transient receptor potential channels in the ependymal cells of the developing rat brain

• Jo KD
• Lee KS
• Lee WT
• Hur MS
• Kim HJ

• KMID: 2046759
• DOI: http://doi.org/10.5115/acb.2013.46.1.68

Abstract

Cerebrospinal fluid (CSF) plays an important role in providing brain tissue with a stable internal environment as well as in absorbing mechanical and thermal stresses. From its initial composition, derived from the amniotic fluid trapped by the closure of neuropores, CSF is modified by developing and differentiating ependymal cells lining the ventricular surface or forming the choroid plexus. Its osmolarity and ionic composition brings about a change through the action of many channels expressed on the ependymal cells. Some newly discovered transient receptor potential (TRP) channels are known to be expressed in the choroid plexus ependyma. To detect additional TRP channel expression, immunohistochemical screening was performed at the choroid plexus of 13-, 15-, 17-, and 19-day embryos, using antibodies against TRPV1, TRPV3, and TRPA1, and the expression was compared with those in the adult TRP channels. The level of TRP channel expression was higher in the choroid plexus which suggests more active functioning of TRP channels in the developing choroid plexus than the ventricular lining ependyma in the 15- and 17-day embryos. All the expression of TRP channels decreased at the 19th day of gestation. TRPA1 was expressed at a higher level than TRPV1 and TRPV3 in almost all stages in both the choroid plexus and ventricular lining epithelium. The highest level of TRPV1 and TRPV3 expression was observed in association with the glycogen deposits in the cytoplasm of the choroid plexus ependymal cells of the 15- and 17-day embryos.

Keyword

Ependyma; Choroid plexus; Transient receptor potential channels; Embryonic development

MeSH Terms

Adult
Amniotic Fluid
Animals
Antibodies
Brain
Choroid Plexus
Cytoplasm
Embryonic Development
Embryonic Structures
Ependyma
Epithelium
Female
Glycogen
Humans
Mass Screening
Osmolar Concentration
Pregnancy
Rats
Transient Receptor Potential Channels
Antibodies
Glycogen
Transient Receptor Potential Channels