Korean Lepr Bull.  2005 Dec;38(2):3-14.

Cloning of Danio rerio toll-like receptor 1

Affiliations
  • 1Department of Dermatology, College of Medicine, Ewha Womans University, Seoul, Korea. hychoi@ewha.ac.kr

Abstract

Toll-like receptors (TLRs) play a central role of innate immunity as well as of enhancing the adaptive immunity. TLR1 interacts with TLR2 to recognize the mycobacterial 19-kD lipoprotein. Danio rerio genomic sequences predicted TLR family genes. Danio rerio TLR1 gene was cloned with RACE-PCR and RT-PCR methods to get more sights of immunity against mycobacterial infection such as leprosy. Cloned TLR1 showed insertion of 22 amino acid residues comparing to NCBI- predicted TLR1, and an open reading frame composing of deduced 795 amino acids. Toll-interlukin1 receptor domain and leucine-rich repeats were in the cloned TLR1 as were noted in NCBI-predicted TLR1. Cloned zebrafish TLR1 had high similarity to human TLR1 with 35% amino acid identities and 57% amino acid positivities across the entire sequence (E=1e-130). The cloned sequence might help to study the innate and adaptive immunity of mycobacterial infection.

Keyword

Cloning; Danio rerio; toll-like receptor 1; leprosy
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