Korean J Occup Environ Med.
2000 Jun;12(2):209-217.
Autoproliferation of Fibroblast by Exposure to Crystalline Silica - Evaluation by H2O2 and PDGF-AA and TGF beta
- Affiliations
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- 1Department of Occupational and Environmental Medicine, St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Korea. nglim@cmc.cuk.ac.kr
Abstract
OBJECTIVES
The aim of this study is to find out the activity of autoproliferation of ratfibroblast exposed to crystalline silica and the role of mediators secreted from rat fibroblast.
METHODS
The effect of alpha-quartz on production of growth factor (platelet-derived growth factor-AA and transforming growth factor beta)from rat fibroblasts were evaluated by ELISA and immunocytochemical analysis. Gene expression of these growth factors in rat fibrobast exposed to crystalline silica was evaluated by RT-PCR. Furthermore, fibroblast proliferation by culture supernatant of rat fibroblast was assayed by the neutral red test.
RESULTS
The amounts of H2O2 and growth factors synthesized in rat fibroblasts were significantly increased by the stimulation of crystalline silica(alpha-quartz), which showed the dose-dependent manner to the concentration of alpha-quartz with the maximum response at the dosage of 100 microgram/cm2. The result of RT-PCR demonstrated that alpha-quartz induced gene expression of PDGF-AA and TGFbeta in rat fibroblast. We also found that supernatant of alpha-quartz-cocultured rat fibroblast induced a significant proliferation of fibroblast.
CONCLUSION
Crystalline silica directly induce functional change in fibroblast such as increased release of reactive oxygen species and growth factors. The products of these functional change promote fibroblast proliferation via autocrine loop.
Keyword
MeSH Terms
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Animals
Crystallins*
Enzyme-Linked Immunosorbent Assay
Fibroblasts*
Gene Expression
Intercellular Signaling Peptides and Proteins
Neutral Red
Rats
Reactive Oxygen Species
Silicon Dioxide*
Transforming Growth Factor beta
Transforming Growth Factors
Crystallins
Intercellular Signaling Peptides and Proteins
Neutral Red
Reactive Oxygen Species
Silicon Dioxide
Transforming Growth Factor beta
Transforming Growth Factors
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